Overview

  • Product nameAnti-UPF3A antibody
    See all UPF3A primary antibodies
  • Description
    Mouse monoclonal to UPF3A
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment: TGGGKQESCA PGAVVKARPM EGSLEEPQET SHSGSDKEHR DVERSQEQES EAQRYHVDDG RRHRAHHEPE RLSRRSEDEQ RWGKGPGQDR GKKGSQDSGA P, corresponding to amino acids 274-375 of Human UPF3A (AAH23569) with tag.

  • Positive control
    • UPF3A transfected 293T cell lysate; Recombinant tagged human UPF3A fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionPart of a multiprotein post-splicing mRNP complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Binds spliced mRNA upstream of exon-exon junctions.
  • Tissue specificityIsoform 1 is strongly expressed in testis, uterus, muscle, fetal brain and spinal cord. Isoform 2 is strongly expressed in fetal brain and spinal cord.
  • Sequence similaritiesBelongs to the RENT3 family.
  • Cellular localizationNucleus. Cytoplasm. Shuttling between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2600001C03Rik antibody
    • 4930546M19Rik antibody
    • hUpf3 antibody
    • HUPF3A antibody
    • MGC151365 antibody
    • Nonsense mRNA reducing factor 3A antibody
    • OTTHUMP00000018789 antibody
    • Regulator of nonsense transcripts 3A antibody
    • REN3A_HUMAN antibody
    • RENT3A antibody
    • Up frameshift suppressor 3 homolog A antibody
    • Up-frameshift suppressor 3 homolog A antibody
    • UPF3 A antibody
    • UPF3 antibody
    • UPF3 regulator of nonsense transcripts homolog A antibody
    • upf3a antibody
    see all

Anti-UPF3A antibody images

  • Anti-UPF3A antibody (ab77423) at 1 µg/ml + Recombinant tagged human UPF3A fragment at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 51 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-UPF3A antibody (ab77423) at 1 µg/ml

    Lane 1 : UPF3A transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 51 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-UPF3A antibody (ab77423)

ab77423 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"