Anti-UPF3B/RENT3B antibody (ab134566)
Key features and details
- Rabbit polyclonal to UPF3B/RENT3B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UPF3B/RENT3B antibody
See all UPF3B/RENT3B primary antibodies -
Description
Rabbit polyclonal to UPF3B/RENT3B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Bat -
Immunogen
Synthetic peptide corresponding to Human UPF3B/RENT3B aa 300-400.
Database link: Q9BZI7 -
Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is independent of association with UPF2 and components of the EJC core. -
Tissue specificity
Expressed in testis, uterus, prostate, heart, muscle, brain, spinal cord and placenta. -
Involvement in disease
Mental retardation, X-linked, syndromic, 14 -
Sequence similarities
Belongs to the RENT3 family. -
Cellular localization
Nucleus. Cytoplasm. Shuttling between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 65109 Human
- Omim: 300298 Human
- SwissProt: Q9BZI7 Human
- Unigene: 103832 Human
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Alternative names
- hUpf3B antibody
- hUpf3p X antibody
- hUpf3p-X antibody
see all
Images
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All lanes : Anti-UPF3B/RENT3B antibody (ab134566) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 10 seconds -
Detection of UPF3B/RENT3B by Western Blot of Immunprecipitate.
Immunoprecipitation of HeLa whole cell lysate using ab134566 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of UPF3B/RENT3B with ab134566 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab134566 has been referenced in 2 publications.
- Palma M et al. A role for AKT1 in nonsense-mediated mRNA decay. Nucleic Acids Res 49:11022-11037 (2021). PubMed: 34634811
- Fontaine KA et al. The Cellular NMD Pathway Restricts Zika Virus Infection and Is Targeted by the Viral Capsid Protein. MBio 9:N/A (2018). PubMed: 30401782