Overview

  • Product nameAnti-UPF3B/RENT3B antibody
    See all UPF3B/RENT3B primary antibodies
  • Description
    Rabbit polyclonal to UPF3B/RENT3B
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: MKEEKEHRPK EKRVTLLTPA GATGSGGGTS GDSSKGEDKQ DRNKEKKEAL, corresponding to N terminal amino acids 1-50 of Human UPF3B/RENT3B

  • Positive control
    • Jurkat cells. Stomach.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • RelevanceUPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. UPF3B binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X (referenced from entrez gene).
  • Cellular localizationNucleus. Cytoplasm. Note: Shuttling between the nucleus and the cytoplasm.
  • Database links
  • Alternative names
    • HUPF3B antibody
    • hUpf3p X antibody
    • MRXS14 antibody
    • Nonsense mRNA reducing factor 3B antibody
    • Regulator of nonsense transcripts 3B antibody
    • RENT3B antibody
    • Up-frameshift suppressor 3 homolog B antibody
    • Up-frameshift suppressor 3 homolog on chromosome X antibody
    • UPF3 regulator of nonsense transcripts homolog B (yeast) antibody
    • UPF3 regulator of nonsense transcripts homolog B antibody
    • UPF3B antibody
    • UPF3X antibody
    see all

Anti-UPF3B/RENT3B antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling UPF3B/RENT3B with ab66753 at 4-8µg/ml. Arrows indicate positively labeling epithelial cells of the fundic gland. Magnification: 400X.

  • Anti-UPF3B/RENT3B antibody (ab66753) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References for Anti-UPF3B/RENT3B antibody (ab66753)

ab66753 has not yet been referenced specifically in any publications.

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