Overview

  • Product nameAnti-UPF3B/RENT3B antibody
    See all UPF3B/RENT3B primary antibodies
  • Description
    Rabbit polyclonal to UPF3B/RENT3B
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 251-300 (KIDRIPERDK LKDEPKIKVH RFLLQAVNQK NLLKKPEKGD EKELDKREKA) of Human UPF3B/RENT3B (NP_542199)

  • Positive control
    • Fetal heart lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceUPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. UPF3B binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X (referenced from entrez gene).
  • Cellular localizationNucleus. Cytoplasm. Note: Shuttling between the nucleus and the cytoplasm.
  • Database links
  • Alternative names
    • HUPF3B antibody
    • hUpf3p X antibody
    • MRXS14 antibody
    • Nonsense mRNA reducing factor 3B antibody
    • Regulator of nonsense transcripts 3B antibody
    • RENT3B antibody
    • Up-frameshift suppressor 3 homolog B antibody
    • Up-frameshift suppressor 3 homolog on chromosome X antibody
    • UPF3 regulator of nonsense transcripts homolog B (yeast) antibody
    • UPF3 regulator of nonsense transcripts homolog B antibody
    • UPF3B antibody
    • UPF3X antibody
    see all

Anti-UPF3B/RENT3B antibody images

  • Anti-UPF3B/RENT3B antibody (ab83249) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)

References for Anti-UPF3B/RENT3B antibody (ab83249)

ab83249 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"