Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab30965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionFunctions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.
  • Tissue specificityExpressed in adrenal tissue, JEG-3, NCI-H295A, HepG2 and HeLa cell lines.
  • Sequence similaritiesBelongs to the grh/CP2 family. CP2 subfamily.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cp2b antibody
    • DKFZp686L1745 antibody
    • LBP 1a antibody
    • LBP 1B antibody
    • LBP1 antibody
    • LBP1A antibody
    • LBP1B antibody
    • NF2d9 antibody
    • Transcription factor LBP-1 antibody
    • UBIP1_HUMAN antibody
    • UBP1 antibody
    • Upstream binding protein 1 antibody
    • Upstream-binding protein 1 antibody
    see all

Anti-Upstream Binding Protein 1 antibody images

  • Anti-Upstream Binding Protein 1 antibody (ab30965) at 0.5 µg/ml + Daudi cell lysate at 10 µg/ml

    Secondary
    HRP conjugated anti-rabbit IgG. at 1/50000 dilution

    Predicted band size : 60 kDa

References for Anti-Upstream Binding Protein 1 antibody (ab30965)

This product has been referenced in:
  • Chul Kang H  et al. PIAS1 regulates CP2c localization and active promoter complex formation in erythroid cell-specific {alpha}-globin expression. Nucleic Acids Res 38:5456-71 (2010). WB ; Human . Read more (PubMed: 20421208) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cell)
Permeabilization Yes - Tween-20
Specification HeLa cell
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Jun 08 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (MEL, K562 cell)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 20 µg
Specification MEL, K562 cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 08 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample Human Tissue sections (human breast carcinoma)
Specification human breast carcinoma
Permeabilization Yes - tween-20
Fixative Paraformaldehyde
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Submitted Jan 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"