Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)
- Datasheet
- Specific References (75)
- Protocols
Overview
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Product nameAnti-UQCRC2 antibody [13G12AF12BB11]
See all UQCRC2 primary antibodies -
DescriptionMouse monoclonal [13G12AF12BB11] to UQCRC2
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Host speciesMouse
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Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, WBmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Human
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Immunogen
Tissue, cells or virus corresponding to Cow UQCRC2.
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Positive control
- Human, bovine, murine and rat heart mitochondria. HepG2 cell lysate.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone number13G12AF12BB11
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab14745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IF | Use a concentration of 0.1 - 0.5 µg/ml. Requires heat-induced antigen retrieval where aldehydes are used as fixatives. |
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| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | 1/1500. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 49.5 kDa. |
Target
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FunctionThis is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
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Sequence similaritiesBelongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
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Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 282394 Cow
- Entrez Gene: 7385 Human
- Entrez Gene: 67003 Mouse
- Entrez Gene: 293448 Rat
- Omim: 191329 Human
- SwissProt: P23004 Cow
- SwissProt: P22695 Human
- SwissProt: Q9DB77 Mouse
see all -
Alternative names
- Complex III subunit 2 antibody
- Core protein II antibody
- Cytochrome b c1 complex subunit 2 mitochondrial antibody
see all
Images
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![Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)](http://a.abcam.com/ps/products/14/ab14745/Images/ab14745-218352-ab14745wb.jpg)
Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)This image is courtesy of an anonymous AbreviewAnti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 1/1000 dilution + H23 whole cell lysate at 20 µg
Secondary
HRP-conjugated goat anti-mouse IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 48 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minute
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)](http://a.abcam.com/ps/products/14/ab14745/Images/UQCRC2-Primary-antibodies-ab14745-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)IHC image of ab14745 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14745, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)](http://a.abcam.com/ps/products/14/ab14745/Images/UQCRC2-Primary-antibodies-ab14745-4.jpg)
Flow Cytometry - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)Overlay histogram showing HepG2 cells stained with ab14745 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14745, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Immunofluorescence - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)](http://a.abcam.com/ps/products/14/ab14745/Images/UQCRC2-Primary-antibodies-ab14745-17.gif)
Immunofluorescence - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)Mitochondrial localization of complex III visualized by immunofluorescence using anti-complex III subunit Core 2 mAb 2E3GC12FB2AE2 (ab14745). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14745 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
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![Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)](http://a.abcam.com/ps/products/14/ab14745/Images/UQCRC2-Primary-antibodies-ab14745-2.jpg)
Western blot - Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)All lanes : Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 0.5 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 48 kDa (why is the actual band size different from the predicted?)
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Protocols
References
This product has been referenced in:
- Zhou C et al. Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth. Cell Death Dis 9:161 (2018). WB . Read more (PubMed: 29415987) »
- Glasgow RIC et al. Novel GFM2 variants associated with early-onset neurological presentations of mitochondrial disease and impaired expression of OXPHOS subunits. Neurogenetics 18:227-235 (2017). WB ; Human . Read more (PubMed: 29075935) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"