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Tissue, cells or virus corresponding to Cow UQCRC2.
This antibody clone is manufactured by Abcam.
Our Abpromise guarantee covers the use of ab14745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IF||Use a concentration of 0.1 - 0.5 µg/ml.
Requires heat-induced antigen retrieval where aldehydes are used as fixatives.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 49.5 kDa.|
This image is courtesy of an anonymous Abreview
Mitochondrial localization of complex III visualized by immunofluorescence using anti-complex III subunit Core 2 mAb 2E3GC12FB2AE2 (ab14745). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14745 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"