Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745)

Overview

  • Product name
    Anti-UQCRC2 antibody [13G12AF12BB11]
    See all UQCRC2 primary antibodies
  • Description
    Mouse monoclonal [13G12AF12BB11] to UQCRC2
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to Cow UQCRC2.

  • Positive control
    • Human, bovine, murine and rat heart mitochondria. HepG2 cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

Applications

Our Abpromise guarantee covers the use of ab14745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IF Use a concentration of 0.1 - 0.5 µg/ml.

Requires heat-induced antigen retrieval where aldehydes are used as fixatives.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/1500.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49.5 kDa.

Target

  • Function
    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
  • Sequence similarities
    Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complex III subunit 2 antibody
    • Core protein II antibody
    • Cytochrome b c1 complex subunit 2 mitochondrial antibody
    • Cytochrome b-c1 complex subunit 2 antibody
    • EC 1.10.2.2 antibody
    • MC3DN5 antibody
    • mitochondrial antibody
    • QCR2 antibody
    • QCR2_HUMAN antibody
    • Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
    • Ubiquinol cytochrome c reductase core protein II antibody
    • Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
    • UQCR2 antibody
    • Uqcrc2 antibody
    see all

Images

  • Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 1/1000 dilution + H23 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • IHC image of ab14745 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14745, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HepG2 cells stained with ab14745 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14745, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Mitochondrial localization of complex III visualized by immunofluorescence using anti-complex III subunit Core 2 mAb 2E3GC12FB2AE2 (ab14745). Cultured human embryonic lung-derived fibroblasts (strain MRC5) were fixed, permeabilized and then labeled with ab14745 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml).

  • All lanes : Anti-UQCRC2 antibody [13G12AF12BB11] (ab14745) at 0.5 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Gómez-Serrano M  et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). WB ; Human . Read more (PubMed: 28064117) »
  • Antonicka H  et al. A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability. EMBO Rep 18:28-38 (2017). Read more (PubMed: 27974379) »

See all 63 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
American Robin (Turdis migratorius) Cell lysate - other (fibroblasts, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18%)
Loading amount
25 µg
Specification
fibroblasts, mitochondrial fraction
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 12 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (AML12 hepatocytes, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
AML12 hepatocytes, mitochondrial fraction
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 22 2017

Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - other (whole fly mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
whole fly mitochondrial fraction
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 21 2017

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% GEL)
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Specification
Mouse Embryonic Fibroblasts
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted May 22 2014

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Human Cultured Cells (H460)
Specification
H460
Permeabilization
Yes - 0.5% Triton-X
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 20 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% GEL)
Sample
Human Cell lysate - whole cell (H23)
Specification
H23
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Feb 17 2014

Thank you for contacting us.

The MSDS for these products have been added to each product datasheet. They should appear on the website within the next few hours (time for the cache to be regenerated).

I hope this information is helpful...

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Thank you for your enquiry.

I can confirm that the following antibodies are produced in our Mitosciences laboratories:


ab14713
ab110244
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ab14745
ab14705

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brown Adipose Tissue)
Specification
Brown Adipose Tissue
Fixative
Paraformaldehyde
Permeabilization
No
Username

Ms. Philippa Hepplewhite

Verified customer

Submitted Aug 03 2012

Thank you for your call earlier.

I now had a look at the homology between the immunogens of the antibodies included in the MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413) and the respective proteins in Drosophila.
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