Overview

  • Product nameAnti-UQCRC2 antibody
    See all UQCRC2 primary antibodies
  • Description
    Rabbit polyclonal to UQCRC2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length Human UQCRC2 (NP_003357.2, amino acids 1-453).

  • Positive control
    • Human colon and Mouse kidney tissue lysates; A431 cell lysate; UQCRC2 transfected 293T cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa

Properties

Applications

Our Abpromise guarantee covers the use of ab103616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionThis is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
  • Sequence similaritiesBelongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complex III subunit 2 antibody
    • Core protein II antibody
    • Cytochrome b c1 complex subunit 2 mitochondrial antibody
    • Cytochrome b-c1 complex subunit 2 antibody
    • EC 1.10.2.2 antibody
    • MC3DN5 antibody
    • mitochondrial antibody
    • QCR2 antibody
    • QCR2_HUMAN antibody
    • Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody
    • Ubiquinol cytochrome c reductase core protein II antibody
    • Ubiquinol-cytochrome-c reductase complex core protein 2 antibody
    • UQCR2 antibody
    • Uqcrc2 antibody
    see all

Anti-UQCRC2 antibody images

  • ICC/IF image of ab103616 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103616 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 48 kDa
  • Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + Mouse kidney tissue lysate at 50 µg

    Predicted band size : 48 kDa
  • Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + A431 cell lysate at 50 µg

    Predicted band size : 48 kDa
  • All lanes : Anti-UQCRC2 antibody (ab103616) at 5 µg/ml

    Lane 1 : UQCRC2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 48 kDa

References for Anti-UQCRC2 antibody (ab103616)

ab103616 has not yet been referenced specifically in any publications.

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