Overview

  • Product name
    Anti-UQCRFS1 antibody [5A5]
    See all UQCRFS1 primary antibodies
  • Description
    Mouse monoclonal [5A5] to UQCRFS1
  • Tested applications
    Suitable for: WB, ICC, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Bovine Complex III

  • Positive control
    • Human, bovine, rat and murine heart mitochondria. In Flow Cytometry, this antibody gave a positive signal in Raji cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab14746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa.
ICC Use a concentration of 5 µg/ml. Requires heat-induced antigen retrieval where aldehydes are used as fixatives.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
    The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.
  • Sequence similarities
    Contains 1 Rieske domain.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complex III subunit 5 antibody
    • Complex III subunit IX antibody
    • Cytochrome b c1 complex subunit Rieske mitochondrial antibody
    • Cytochrome b-c1 complex subunit 11 antibody
    • Cytochrome b-c1 complex subunit 5 antibody
    • Cytochrome b6f complex iron sulfur subunit, chloroplastic antibody
    • petC antibody
    • PGR1 antibody
    • Plastohydroquinone:plastocyanin oxidoreductase iron sulfur protein antibody
    • Proton gradient regulation protein 1 antibody
    • Rieske iron sulfur protein antibody
    • Rieske iron-sulfur protein antibody
    • RIP1 antibody
    • RIS1 antibody
    • RISP antibody
    • Ubiquinol cytochrome C reductase rieske iron sulphur antibody
    • Ubiquinol cytochrome c reductase Rieske iron-sulfur polypeptide 1 antibody
    • Ubiquinol-cytochrome c reductase 8 kDa protein antibody
    • Ubiquinol-cytochrome c reductase iron-sulfur subunit antibody
    • UCRI_HUMAN antibody
    • UQCR5 antibody
    • UQCRFS1 antibody
    see all

Anti-UQCRFS1 antibody [5A5] images

  • All lanes : Anti-UQCRFS1 antibody [5A5] (ab14746) at 0.5 µg/ml

    Lane 1 : Hela Whole Cell Lysate - Hydroxyurea Treated
    Lane 2 : Hela Whole Cell Lysate - Bleomycin Treated
    Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated

    Lysates/proteins at 10 µg per lane.

    Secondary
    Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Human normal kidney (cortex). Staining is observed intracellular. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • All lanes : Anti-UQCRFS1 antibody [5A5] (ab14746) at 1 µg/ml

    Lane 1 : Isolated mitochondria from human heart at 5 µg
    Lane 2 : Isolated mitochondria from bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg
    Lane 4 : Isolated mitochondria from mouse heart at 10 µg
  • Overlay histogram showing Raji cells stained with ab14746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14746, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-UQCRFS1 antibody [5A5] (ab14746)

This product has been referenced in:
  • Stram AR  et al. Progressive mitochondrial protein lysine acetylation and heart failure in a model of Friedreich's ataxia cardiomyopathy. PLoS One 12:e0178354 (2017). WB ; Mouse . Read more (PubMed: 28542596) »
  • Peyta L  et al. Reduced cardiolipin content decreases respiratory chain capacities and increases ATP synthesis yield in the human HepaRG cells. Biochim Biophys Acta 1857:443-53 (2016). Read more (PubMed: 26768115) »

See all 32 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - other (Isolated mitochondria)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Isolated mitochondria
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2017

Application
Western blot
Sample
Mouse Cell lysate - other (various)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
various
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Dec 14 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (flight muscles)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
40 µg
Specification
flight muscles
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 06 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (h9c2 cell)
Loading amount
30 µg
Specification
h9c2 cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Mar 12 2013


ab14746 can be used in a western bot under denaturing conditions i.e using SDS. Indeed our customers have found that this antibody gives good results under reducing and denaturing conditions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA pH8.0
Username

Ms. Philippa Hepplewhite

Verified customer

Submitted Aug 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Gastric mucosa)
Loading amount
20 µg
Specification
Gastric mucosa
Gel Running Conditions
Reduced Denaturing (8-16%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Maria Paola Simula

Verified customer

Submitted Jun 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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