Overview

  • Product nameAnti-UQCRQ antibody [1H9DE5DG5BC8]
    See all UQCRQ primary antibodies
  • Description
    Mouse monoclonal [1H9DE5DG5BC8] to UQCRQ
  • Tested applicationsSuitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Bovine Complex III subunit 8 (UQCRQ)

  • Positive control
    • Human, Cow, Rat or Mouse heart mitochondrial lysate
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/1. Predicted molecular weight: 6 kDa.

Target

  • FunctionThis is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This subunit, together with cytochrome b, binds to ubiquinone.
  • Involvement in diseaseDefects in UQCRQ are a cause of mitochondrial complex III deficiency (MT-C3D) [MIM:124000]. A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected. Clinical features include mitochondrial encephalopathy, psychomotor retardation, ataxia, severe failure to thrive, liver dysfunction, renal tubulopathy, muscle weakness and exercise intolerance.
  • Sequence similaritiesBelongs to the UQCRQ/QCR8 family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complex III subunit 8 antibody
    • Complex III subunit VIII antibody
    • Cytochrome b-c1 complex subunit 8 antibody
    • QCR8 antibody
    • QCR8_HUMAN antibody
    • QP-C antibody
    • QPC antibody
    • Ubiquinol-cytochrome c reductase complex 9.5 kDa protein antibody
    • Ubiquinol-cytochrome c reductase complex ubiquinone-binding protein QP-C antibody
    • Uqcrq antibody
    see all

Anti-UQCRQ antibody [1H9DE5DG5BC8] images

  • Overlay histogram showing HepG2 cells stained with ab110255 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110255, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-UQCRQ antibody [1H9DE5DG5BC8] (ab110255) at 1 µg/ml

    Lane 1 : Human mitochondrial lysate at 10 µg
    Lane 2 : Cow mitochondrial lysate at 1 µg
    Lane 3 : Rat mitochondrial lysate at 10 µg
    Lane 4 : Mouse mitochondrial lysate at 10 µg


    Predicted band size : 6 kDa

References for Anti-UQCRQ antibody [1H9DE5DG5BC8] (ab110255)

This product has been referenced in:
  • Pérez-Pérez R  et al. COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation. Cell Rep 16:2387-98 (2016). WB . Read more (PubMed: 27545886) »
  • Mineri R  et al. How do human cells react to the absence of mitochondrial DNA? PLoS One 4:e5713 (2009). Read more (PubMed: 19492094) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"