Overview

  • Product nameAnti-URB2 antibody
    See all URB2 primary antibodies
  • Description
    Rabbit polyclonal to URB2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to C terminal amino acids 1494-1524 of Human URB2 (NP_055592.2).

  • Positive control
    • MDA-MB453 cell line lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified through a protein A column, followed by peptide affinity purification
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 171 kDa.

Target

  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C77575 antibody
    • KIAA0133 antibody
    • mKIAA0133 antibody
    • NET10 antibody
    • NPA2 antibody
    • nucleolar preribosomal-associated protein 1 antibody
    • RGD1307980 antibody
    • Unhealthy ribosome biogenesis protein 2 homolog antibody
    • URB2 antibody
    • URB2 ribosome biogenesis 2 homolog (S. cerevisiae) antibody
    • URB2_HUMAN antibody
    see all

Anti-URB2 antibody images

  • Anti-URB2 antibody (ab123396) at 1/100 dilution + MDA-MB453 cell line lysates at 35 µg

    Predicted band size : 171 kDa
  • All lanes : Anti-URB2 antibody (ab123396) at 1/1000 dilution

    Lane 1 : Hela extracts 25ug
    Lane 2 : RPE-1 extracts 25ug
    Lane 3 : Hela extracts 50ug
    Lane 4 : RPE-1 extracts 50ug

    Secondary
    Donkey LICOR anti-rabbit IRDye680 at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 171 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa (possible cross reactivity).

    Exposure time : 3 minutes

    See Abreview

References for Anti-URB2 antibody (ab123396)

ab123396 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa and RPE-1)
Loading amount 25 µg
Specification HeLa and RPE-1
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris (MES Buffer))
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"