Overview

  • Product nameUrea Assay Kit
  • Detection methodColorimetric
  • Tests
    100 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Tissue Extracts, Cell Lysate
  • Assay typeQuantitative
  • Sensitivity
    > 0.5 nmol/well
  • Range
    0.5 nmol/well - 5 nmol/well
  • Assay time
    1h 00m
  • Product overview

    Urea Assay Kit (ab83362) provides a rapid, simple, sensitive, and reliable for measurement of Urea level in a variety of samples such as serum, plasma, and urine, etc. In the assay, Urea reacts as substrate with compounds in the presence of enzymes to form a product that reacts with the probe to generate color (ODmax=570nm). The optical density of produced color is directly proportional to the concentration of urea in the solution. The kit can detect as low as 0.5 nmol per well or 10 µM of Urea. The assay is also suitable for high throughput studies.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Urea is a waste product which produced in the liver, dissolved in blood (in a concentration of 2.5-7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons. Urea determination is very useful for the medical clinician to assess kidney and other organs function of patients.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceUrea is a waste product which is produced in the liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons.
  • Alternative names
    • Carbamide
    • Carbonyl diamide
    • Carbonyldiamine

Applications

Our Abpromise guarantee covers the use of ab83362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Urea Assay Kit images

  • Urea measured in cell culture medium and control medium (MEM), background signal subtracted (duplicates +/- SD).

  • Urea measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

  • Urea measured in cell lysates, background signal subtracted (duplicates; +/- SD).

  • Example of Urea standard curve using ab83362

Protocols

References for Urea Assay Kit (ab83362)

This product has been referenced in:
  • Howie RN  et al. A Model for Osteonecrosis of the Jaw with Zoledronate Treatment following Repeated Major Trauma. PLoS One 10:e0132520 (2015). Read more (PubMed: 26186665) »
  • Baumgartner WA  et al. Modulation of vacuolar pH is required for replication of Edwardsiella ictaluri in channel catfish macrophages. Infect Immun 82:2329-36 (2014). Read more (PubMed: 24664505) »

See all 5 Publications for this product

Product Wall

We have not tested urea that has been reacted at both amine groups. However, it will likely not work because the enzyme reaction is very specific for urea.

The phenol red could interfere if the amount of medium used is enough to contribute red color to the sample. Typically when diluted medium is used and the volume is made up to 50ul with buffer, the color does not interfere in the assay. Since the probe...

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For all samples, including tissue or cell extracts, add 1-25ul sample to each well and volume up to 50ul with Assay Buffer.

Add 50 ul Urea Reaction Mix to each sample or standard.

Final volume in each well will be ...

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To answer your questions:

1) The desired wavelength for reading the samples is 570 nm.

If the detection band width on the plate reader is ± 30, then a wavelength of ± 30 of 570 can be used, which is 540-600 nm. Othe...

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Thank you for contacting us.

The calculation is right. We suggest in the protocol diluting the standard to get 0.5milli molar per 1000ul.

This 0.5 is a molar solution means, it is 0.5 milli mol per litre per 1000ul i.e. 0.5 nano mol ...

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Thank you for contacting us.

I have double checked your question with the laboratory, and I am happy to confirm, that this kit should work with the buffers your client mentions. It will work with all common lab buffers. The only ones it woul...

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Thank you for your enquiry regarding ab83362.
If the detection band width on the plate reader is ± 30, then a wavelength of ± 30 of 570 can be used, which is 540-600 nm. Otherwise the efficiency of detection of this assay will decrease.
Unfor...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to ...

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Thanks for your reply.

RE: "As the item did not come in on dry ice, the storage condition is already compromised over the shipment."

This is not the case. Components of this kit are stable when shipped overnight in the man...

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Answer re: Q1: The kit components provide maximum efficiency only when stored at the right temp. There is a possibility of the kit component’s’ quality being compromised, resulting in sub-standard results.




Answer ...

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1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"