Urea Assay Kit (ab83362) provides a rapid, simple, sensitive, and reliable for measurement of Urea level in a variety of samples such as serum, plasma, and urine, etc. In the assay, Urea reacts as substrate with compounds in the presence of enzymes to form a product that reacts with the probe to generate color (ODmax=570nm). The optical density of produced color is directly proportional to the concentration of urea in the solution. The kit can detect as low as 0.5 nmol per well or 10 µM of Urea. The assay is also suitable for high throughput studies.
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Urea is a waste product which produced in the liver, dissolved in blood (in a concentration of 2.5-7.5 mM), and secreted by the kidneys. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body, and the countercurrent system which allows for reabsorption of water and critical ions in the nephrons. Urea determination is very useful for the medical clinician to assess kidney and other organs function of patients.
|Converting Enzyme (Lyophilised)||Blue||1 vial|
|Developer (Lyophilised)||Orange||1 vial|
|Enzyme Mix (lyophilized)||Green||1 vial|
|OxiRed Probe in DMSO||Red||1 x 200µl|
|Urea Assay Buffer||WM||1 x 25ml|
|Urea Standard (100mM)||Yellow||1 x 100µl|
Our Abpromise guarantee covers the use of ab83362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Urea measured in cell culture medium and control medium (MEM), background signal subtracted (duplicates +/- SD).
Urea measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).
Urea measured in cell lysates, background signal subtracted (duplicates; +/- SD).