Anti-Urm1 antibody (ab4755)

Overview

  • Product nameAnti-Urm1 antibody
    See all Urm1 primary antibodies
  • Description
    Rabbit polyclonal to Urm1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein (S. cerevisiae).

  • Positive control
    • Most yeast cell lysates can be used as a positive control without induction or stimulation.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 12 kDa.
ELISA 1/2000 - 1/10000.

Target

  • FunctionActs as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.
  • PathwaytRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
  • Sequence similaritiesBelongs to the URM1 family.
  • Post-translational
    modifications
    C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
    • Alternative names
      • 2900073H19Rik antibody
      • C9orf74 antibody
      • Chromosome 9 open reading frame 74 antibody
      • MGC2668 antibody
      • RGD1306599 antibody
      • RP11 339B21.4 antibody
      • RP23-443G7.1 antibody
      • Ubiquitin related modifier 1 antibody
      • Ubiquitin related modifier 1 homolog antibody
      • Ubiquitin-related modifier 1 homolog antibody
      • Urm 1 antibody
      • URM1 antibody
      • URM1_HUMAN antibody
      see all

    Anti-Urm1 antibody images

    • ab4755 was tested by immunoblot against yeast lysates expressing the Urm1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1/1000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1/15000 dilution of Donkey polyclonal to Goat IgG (HRP)(ab7125). Panel C shows specific reaction with Urm1 using a 1/1000 dilution of ab4755 followed by reaction with a 1/15000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Urm1 is highly specific and does not cross react with other UBLs. Bands present in Panel C indicate that Urm1 and conjugated Urm1 is present in most yeast cell lysates albeit at significantly reduced levels relative to the Urm1-GFP transfected lysate. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication.

    References for Anti-Urm1 antibody (ab4755)

    ab4755 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Schizosaccharomyces pombe Cell lysate - whole cell (Yeast cells)
    Loading amount 50 µg
    Specification Yeast cells
    Gel Running Conditions Non-reduced Denaturing
    Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 13 2011

    I am very pleased to hear you would like to accept our offer and test ab4755 in S. pombe. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for S. pombe and include this code in ...

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    Thank you for your enquiry. I was passed your details by Aris in our marketing department. We have two antibodies that may be of interest: ab4755  Anti-Urm1 antibody      ab110699  Anti-Urm1 antibody [EPR4809]   To our knowledge, these antib...

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    Thank you for getting back in touch with me. I have been in touch with the source of this antibody and they have confirmed that this antibody was indeed NOT applied in the Furukawa K et al. 2000 publication. The source of this antibody commented...

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    Thank you for your enquiry. I have been in touch with the source of this antibody and they found it difficult to comment on the differences between those supplied for us on our datasheet and those documented in Furukawa et al., 1999. This antibody ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"