Anti-Urm1 antibody [EPR4809] (ab110699)

Overview

  • Product nameAnti-Urm1 antibody [EPR4809]
    See all Urm1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4809] to Urm1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Urm1.

  • Positive control
    • Human fetal liver, A549, Human fetal lung, HepG2, and DDDDK-tagged Urm1 recombinant protein.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionActs as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.
    • PathwaytRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
    • Sequence similaritiesBelongs to the URM1 family.
    • Post-translational
      modifications
      C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2900073H19Rik antibody
      • C9orf74 antibody
      • Chromosome 9 open reading frame 74 antibody
      • MGC2668 antibody
      • RGD1306599 antibody
      • RP11 339B21.4 antibody
      • RP23-443G7.1 antibody
      • Ubiquitin related modifier 1 antibody
      • Ubiquitin related modifier 1 homolog antibody
      • Ubiquitin-related modifier 1 homolog antibody
      • Urm 1 antibody
      • URM1 antibody
      • URM1_HUMAN antibody
      see all

    Anti-Urm1 antibody [EPR4809] images

    • All lanes : Anti-Urm1 antibody [EPR4809] (ab110699) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate at 10 µg
      Lane 2 : A549 lysate at 10 µg
      Lane 3 : Human fetal lung lysate at 10 µg
      Lane 4 : HepG2 lysate at 10 µg
      Lane 5 : DDDDK-tagged Urm1 recombinant protein at 0.01 µg


      Predicted band size : 11 kDa

    References for Anti-Urm1 antibody [EPR4809] (ab110699)

    ab110699 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your interest in the testing discount program. We may be able to offer the testing discount that you saw mentioned on the Scientific support section of the online datasheet. It will depend on the nature of your assay: western blotting, im...

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    Thank you for your enquiry. I was passed your details by Aris in our marketing department. We have two antibodies that may be of interest: ab4755  Anti-Urm1 antibody      ab110699  Anti-Urm1 antibody [EPR4809]   To our knowledge, these antib...

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