Overview

  • Product nameAnti-Urocortin 3 antibody
    See all Urocortin 3 primary antibodies
  • Description
    Rabbit polyclonal to Urocortin 3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Urocortin 3.

    (Peptide available as ab90638.)

  • Positive control
    • This antibody gave a positive signal in human brain pituitary tissue lysate. IF/ICC: HepG2 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab79121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • RelevanceUrocortin 3 is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. A powerful cytoprotective effect of urocortin peptide administration against ischemia and reperfusion injury has been demonstrated in isolated cardiomyocyte models, as well as in the intact heart both in vitro and in vivo.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • MGC119002 antibody
    • OTTHUMP00000018999 antibody
    • Prepro urocortin 3 antibody
    • SCP antibody
    • SPC antibody
    • Stresscopin antibody
    • Ucn III antibody
    • UCN3 antibody
    • UCN3 urocortin 3 stresscopin antibody
    • UCNIII antibody
    • Urocortin 3 antibody
    • Urocortin 3 stresscopin antibody
    • Urocortin III antibody
    see all

Anti-Urocortin 3 antibody images

  • ICC/IF image of ab79121 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79121, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Urocortin 3 antibody (ab79121) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References for Anti-Urocortin 3 antibody (ab79121)

ab79121 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"