Anti-Uroguanylin antibody (ab52806)

Overview

  • Product nameAnti-Uroguanylin antibody
    See all Uroguanylin primary antibodies
  • Description
    Mouse polyclonal to Uroguanylin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Partial recombinant fusion protein, corresponding to amino acids 97-113 of Human Uroguanylin

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 12 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionEndogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport.
  • Tissue specificityStomach and intestine.
  • Sequence similaritiesBelongs to the guanylin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCAP II antibody
    • GCAP-II antibody
    • Guanylate cyclase activator 2B antibody
    • Guanylate cyclase C-activating peptide II antibody
    • GUC2B_HUMAN antibody
    • GUCA2B antibody
    • UGN antibody
    • Uroguanylin antibody
    see all

Anti-Uroguanylin antibody images

  • All lanes : Anti-Uroguanylin antibody (ab52806) at 1/1000 dilution

    Lane 1 : ~20ug of a total protein extract from E coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen.
    Lane 2 : ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant Uroguanylin fusion protein containing a His tag).

    Secondary
    HRP-conjugated Rabbit anti-mouse IgG + IgM (H+L) at 1/5000 dilution

    Predicted band size : 12 kDa

References for Anti-Uroguanylin antibody (ab52806)

ab52806 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and your interest in our products. The Western blot image contains a test lane of ~20ug of a total protein extract from E. coli with ~50 ng to 500 ng of the antigen (recombinant fusion protein containing a His tag). The ne...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"