Overview

  • Product name
  • Description
    Rabbit polyclonal to Urokinase
  • Specificity
    This antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa). This antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase.
  • Tested applications
    Suitable for: ICC/IF, WB, Dot blot, IHC-Fr, IHC-P, ELISA, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human). Extensively purified native HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from human urine.

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: DU145.

Properties

Applications

Our Abpromise guarantee covers the use of ab24121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
Dot blot Use at an assay dependent concentration.
IHC-Fr 1/50.
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
RIA Use at an assay dependent concentration.

Target

  • Function
    Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificity
    Expressed in the prostate gland and prostate cancers.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATF antibody
    • ATF uPA antibody
    • BDPLT5 antibody
    • Plasminogen activator antibody
    • Plasminogen activator urinary antibody
    • Plasminogen activator urokinase antibody
    • PLAU antibody
    • QPD antibody
    • u PA antibody
    • U plasminogen activator antibody
    • u-PA antibody
    • U-plasminogen activator antibody
    • uPA antibody
    • URK antibody
    • UROK_HUMAN antibody
    • Urokinase plasminogen activator antibody
    • Urokinase type plasminogen activator antibody
    • Urokinase type plasminogen activator precursor antibody
    • Urokinase-type plasminogen activator chain B antibody
    see all

Images

  • ICC/IF image of ab24121 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24121 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab24121 at 1/200 dilution staining HMW-scuPA in human brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 10% serum for 12 hours at room temperature and then incubated with ab24121, for 18 hours at 4°C. A goat monoclonal to rabbit Ig, diluted 1/50, was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

See all 11 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Purified protein (human kidney cell)
Loading amount
1 µg
Specification
human kidney cell
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: EDTA
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Mar 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up