Overview

  • Product nameAnti-Urokinase antibody
    See all Urokinase primary antibodies
  • Description
    Rabbit polyclonal to Urokinase
  • SpecificityThis antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa). This antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase.
  • Tested applicationsSuitable for: ICC/IF, WB, Dot blot, IHC-Fr, IHC-P, ELISA, RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human). Extensively purified native HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from human urine.

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: DU145.

Properties

Applications

Our Abpromise guarantee covers the use of ab24121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
Dot blot Use at an assay dependent concentration.
IHC-Fr 1/50.
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
RIA Use at an assay dependent concentration.

Target

  • FunctionSpecifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificityExpressed in the prostate gland and prostate cancers.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATF antibody
    • ATF uPA antibody
    • BDPLT5 antibody
    • Plasminogen activator antibody
    • Plasminogen activator urinary antibody
    • Plasminogen activator urokinase antibody
    • PLAU antibody
    • QPD antibody
    • u PA antibody
    • U plasminogen activator antibody
    • u-PA antibody
    • U-plasminogen activator antibody
    • uPA antibody
    • URK antibody
    • UROK_HUMAN antibody
    • Urokinase plasminogen activator antibody
    • Urokinase type plasminogen activator antibody
    • Urokinase type plasminogen activator precursor antibody
    • Urokinase-type plasminogen activator chain B antibody
    see all

Anti-Urokinase antibody images

  • ICC/IF image of ab24121 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24121 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab24121 at 1/200 dilution staining HMW-scuPA in human brain tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 10% serum for 12 hours at room temperature and then incubated with ab24121, for 18 hours at 4°C. A goat monoclonal to rabbit Ig, diluted 1/50, was used as the secondary antibody.

    See Abreview

References for Anti-Urokinase antibody (ab24121)

This product has been referenced in:
  • Wang L  et al. uPA binding to PAI-1 induces corneal myofibroblast differentiation on vitronectin. Invest Ophthalmol Vis Sci 53:4765-75 (2012). WB ; Human . Read more (PubMed: 22700714) »
  • Lash GE  et al. Regulation of extravillous trophoblast invasion by uterine natural killer cells is dependent on gestational age. Hum Reprod 25:1137-45 (2010). Read more (PubMed: 20219775) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (human kidney cell)
Loading amount 1 µg
Specification human kidney cell
Gel Running Conditions Reduced Denaturing (12 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: EDTA
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Mar 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"