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Extensively purified HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from urine (Human).
ab24122 binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is also inhibited. Human whole plasma or plasma proteins do not interfere with its binding to urokinase.
Our Abpromise guarantee covers the use of ab24122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||Use 10µg for 10 cells.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 54, 52 , 33 kDa.|
|RIA||Use at an assay dependent concentration.|
ICC/IF image of ab24122 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA /0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24122 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab24122 has not yet been referenced specifically in any publications.