Anti-Urokinase antibody [PGM2001] (ab8475)

Overview

  • Product nameAnti-Urokinase antibody [PGM2001]
    See all Urokinase primary antibodies
  • Description
    Mouse monoclonal [PGM2001] to Urokinase
  • SpecificityThis antibody is highly specific for urokinase. No cross reactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). This antibody binds to human high molecular weight single-chain urokinase type plasminogen activator (HMW-scuPA, 54 kDa), to high molecular weight two-chain urokinase type plasminogen activator (HMW-tcuPA, 52 kDa) and to low molecular weight single-chain urokinase type plasminogen activator (LMW-scuPA, 33 kDa) with high affinity (Kd= 0.1 nM) It does not inhibit the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is not inhibited. Human whole plasma or plasma proteins do not interfere with this antibodies binding to urokinase.
  • Tested applicationsSuitable for: Other, Dot blot, ELISA, IHC-Fr, RIA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Urinary Urokinase (Human).

  • General notesMethod of purification: PEG6000 precipitation + DEAE cellulose chromatography, gradient elution. The buffer composition before lyophilisation is PBS with manitol. After reconstitution the concentration is 0.2 mg/mL.

Properties

Applications

Our Abpromise guarantee covers the use of ab8475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Other Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
AP Use at an assay dependent concentration.
ELISA 1/5000. This antibody can be used for quantitative, specific and sensitive assay for urokinase in human fluids (RIA, ELISA) in combination with the non-inhibitory antibody ab8473.This antibody is very stable and can, after appropriate binding, be used over 20-40 times for immunopurification of urokinase in 1 M guanidine chloride.
IHC-Fr 1/30 - 1/50.
RIA Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml.

Target

  • FunctionSpecifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificityExpressed in the prostate gland and prostate cancers.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATF antibody
    • ATF uPA antibody
    • BDPLT5 antibody
    • Plasminogen activator antibody
    • Plasminogen activator urinary antibody
    • Plasminogen activator urokinase antibody
    • PLAU antibody
    • QPD antibody
    • u PA antibody
    • U plasminogen activator antibody
    • u-PA antibody
    • U-plasminogen activator antibody
    • uPA antibody
    • URK antibody
    • UROK_HUMAN antibody
    • Urokinase plasminogen activator antibody
    • Urokinase type plasminogen activator antibody
    • Urokinase type plasminogen activator precursor antibody
    • Urokinase-type plasminogen activator chain B antibody
    see all

References for Anti-Urokinase antibody [PGM2001] (ab8475)

ab8475 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lung epithelial cells)
Loading amount 25 µg
Specification lung epithelial cells
Gel Running Conditions Reduced Denaturing (8)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Urine)
Loading amount 10 µg
Specification Urine
Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Dec 09 2009

Thank you for your enquiry. I am afraid that your customer might have received the product in its original state and I sincerely apologize for the mix-up. However, the product is still viable. This product is supplied as 0.2 mg purified lyophil...

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Thank you for your enquiryand your patience. I have the same answer for this antibody. The buffer composition before lyophilization is PBS with manitol. After reconstitution the IgG concentration is 0.2 mg/mL. I hope this information helps, ple...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"