Anti-Urokinase antibody [U-6] (ab82250)

Overview

  • Product nameAnti-Urokinase antibody [U-6]
    See all Urokinase primary antibodies
  • Description
    Mouse monoclonal [U-6] to Urokinase
  • Specificityab82250 binds single and two-chain Urokinase, uPA/PAI-1 complex and uPA/uPAR complex. A reaction is seen with the amino terminal fragment (ATF) but not with LMW Urokinase. ab82250 reacts with reduced and non-reduced Urokinase in Western blotting. It inhibits the enzymatic activity of Urokinase in the fibrin plate assay and can be used on frozen sections in immunohistochemistry applications. ab82250 is specific for human Urokinase. No reaction with human tissue plasminogen activator (tPA) is seen when tested by ELISA, immunoblotting and enzyme inhibition. No reaction with any other human plasma proteins is seen when tested by immunoblotting.
  • Tested applicationsSuitable for: WB, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native human Urokinase

  • Epitopeab82250 binds to the A chain of Urokinase.

Properties

Applications

Our Abpromise guarantee covers the use of ab82250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
IHC-Fr 1/25.
ELISA Use at an assay dependent concentration.

Target

  • FunctionSpecifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificityExpressed in the prostate gland and prostate cancers.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATF antibody
    • ATF uPA antibody
    • BDPLT5 antibody
    • Plasminogen activator antibody
    • Plasminogen activator urinary antibody
    • Plasminogen activator urokinase antibody
    • PLAU antibody
    • QPD antibody
    • u PA antibody
    • U plasminogen activator antibody
    • u-PA antibody
    • U-plasminogen activator antibody
    • uPA antibody
    • URK antibody
    • UROK_HUMAN antibody
    • Urokinase plasminogen activator antibody
    • Urokinase type plasminogen activator antibody
    • Urokinase type plasminogen activator precursor antibody
    • Urokinase-type plasminogen activator chain B antibody
    see all

References for Anti-Urokinase antibody [U-6] (ab82250)

ab82250 has not yet been referenced specifically in any publications.

Product Wall

This antibody ispurified from cell supernatant.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"