Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit (ab108915)

Overview

  • Product nameUrokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit
    See all Urokinase type Plasminogen Activator kits
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Plasma
  • Assay typeQuantitative
  • Sensitivity
    < 0.006 U/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    Urokinase type plasminogen activator (uPA) is a highly restricted serine protease that converts the zymogen plasminogen to active plasmin, a broad-spectrum serine proteinase capable of degrading most of the major protein components of the extracellular matrix. Binding of uPA to its receptor and subsequent uPA mediated pericellular proteolysis are involved in many process including cell migration and tissue remodelling in angiogenesis, atherogenesis, tumor cell metastasis, and ovulation. High level of uPA is a poor prognostic marker for aggressive breast cancer, aggressive prostate cancer, bladder cancer and gastric cancer.

    ab108915 uPA Human Chromogenic Activity Assay Kit is developed to determine Human uPA activity in plasma and cell culture supernatants. The assay measures the ability of uPA to activate the plasminogen to plasmin in coupled or indirect assays that contain uPA, plasminogen, and a plasmin-specific synthetic substrate. The amount of plasmin produced is quantitated using a highly specific plasmin substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA in the reaction solution at 405 nm is directly proportional to the uPA enzymatic activity.

     

  • Tested applicationsSuitable for: Indirect ELISAmore details

Properties

  • FunctionSpecifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
  • Tissue specificityExpressed in the prostate gland and prostate cancers.
  • Involvement in diseaseQuebec platelet disorder
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • ATF
    • BDPLT5
    • EC 3.4.21
    • EC 3.4.21.73
    • Plasminogen Activator, Urinary
    • Plasminogen Activator, Urokinase
    • PLAU
    • QPD
    • U-PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase-Type Plasminogen Activator
    • Urokinase-type plasminogen activator chain B
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit images

  • uPA measured in biologicals showing quantity (IU) per mL of sample tested.

  • uPA Chromogenic Activity Standard Curve (For Low uPA Activity Samples)
  • uPA Chromogenic Activity Standard Curve (For High uPA Activity Samples)

Protocols

References for Urokinase type Plasminogen Activator Human Chromogenic Activity Assay Kit (ab108915)

ab108915 has not yet been referenced specifically in any publications.

Product Wall



For uPA activity kits, we have two types of kit. One is ab108916 which is direct using uPA substrate to generate the color. The uPA substrate is specifically designed for its specificity for uPA. This assay can differentiate uPA activity vs. ...

Read More

Thank you for your enquiry.

I can confirm the pH for the assay diluent buffer is 8,5.

I hope this will be helpful. If you require any further details or information, please donot hesitate to let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"