Anti-uronyl 2 sulfotransferase antibody (ab65555)

Overview

  • Product nameAnti-uronyl 2 sulfotransferase antibody
    See all uronyl 2 sulfotransferase primary antibodies
  • Description
    Rabbit polyclonal to uronyl 2 sulfotransferase
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog
  • Immunogen

    A region within a synthetic peptide: DVLLLLERFL PHYFKGVLSI YKDPEHRKLG NMTVTVKKTV PSPEAVQILY, corresponding to C terminal amino acids 289-338 of Human uronyl 2 sulfotransferase

  • Positive control
    • Hepg2 cell lysate and human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

Anti-uronyl 2 sulfotransferase antibody images

  • Anti-uronyl 2 sulfotransferase antibody (ab65555) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab65555 at 4µg/ml staining uronyl 2 sulfotransferase in epithelial cells of human kidney renal tubule by Immunohistochemistry, Paraffin-embedded tissue.

References for Anti-uronyl 2 sulfotransferase antibody (ab65555)

ab65555 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"