Anti-Uroplakin Ia antibody - C-terminal (ab95207)

Overview

  • Product name
    Anti-Uroplakin Ia antibody - C-terminal
    See all Uroplakin Ia primary antibodies
  • Description
    Rabbit polyclonal to Uroplakin Ia - C-terminal
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the C-terminal region of human Uroplakin Ia conjugated to KLH (NP_008931.1).

  • Positive control
    • K562 cell line lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab95207 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 29 kDa.

Target

  • Relevance
    This protein is a member of the transmembrane 4 superfamily, which are cell-surface proteins characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.
  • Cellular localization
    Plasma membrane
  • Database links
  • Alternative names
    • MGC129751 antibody
    • MGC129752 antibody
    • MGC14388 antibody
    • TSPAN21 antibody
    • UP1A antibody
    • UPIA antibody
    • UPKA antibody
    see all

Images

  • Anti-Uroplakin Ia antibody - C-terminal (ab95207) at 1/100 dilution + K562 cell line lysates at 35 µg

    Predicted band size : 29 kDa

References

This product has been referenced in:
  • Osborn SL  et al. Induction of human embryonic and induced pluripotent stem cells into urothelium. Stem Cells Transl Med 3:610-9 (2014). Human . Read more (PubMed: 24657961) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Bladder carcinoma)
Permeabilization
No
Specification
Bladder carcinoma
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraffin
Username

Abcam user community

Verified customer

Submitted Aug 03 2017

Merci de votre émail.

Veuillez trouver ci joint le protocole du laboratoire qui avait testé cet anticorps en Western blot.

J'espère que ceci va aider à résoudre le problème. Veuillez nous te...

Read More

Merci pour votre patience.

Malheureusement le laboratoire ne peux pas non plus expliquer cette bande. Ils avaient testé cet anticorps aussi sur la lignée cellulaire CEM avec des bandes similaires que sur l'image sur notre fiche ...

Read More

Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée que vous avez eu des difficultés à obtenir de bons résultats avec ab95207.

J'ai contacté ...

Read More

Je suis désolée d'apprendre que ab95207 ne vous donne pas de bons résultats en Western blot.

Veuillez trouver ci-jointlequestionnaire discuté au téléphone qui nous permet de regrouper le plus d'infor...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up