Anti-Uroplakin Ib antibody (ab102961)

Overview

  • Product nameAnti-Uroplakin Ib antibody
    See all Uroplakin Ib primary antibodies
  • Description
    Rabbit polyclonal to Uroplakin Ib
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 166-196 of Human Uroplakin Ib

  • Positive control
    • K562 cell lysate, Human Brain Tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab102961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 30 kDa.
IHC-P 1/50 - 1/100.

Target

  • FunctionComponent of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.
  • Tissue specificityBladder epithelium.
  • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications
    N-glycosylated with high-mannose oligosaccharides.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tetraspanin-20 antibody
    • Tspan-20 antibody
    • TSPAN20 antibody
    • UP1b antibody
    • UPIb antibody
    • UPK1B antibody
    • UPK1B_HUMAN antibody
    • Uroplakin Ib antibody
    • Uroplakin-1b antibody
    see all

Anti-Uroplakin Ib antibody images

  • Anti-Uroplakin Ib antibody (ab102961) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 30 kDa
  • Immunohistochemical staining of formalin fixed and paraffin embedded brain tissue followed by HRP-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of K562 cells (green histogram) using ab102961 compared to a negative control cell (blue histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

References for Anti-Uroplakin Ib antibody (ab102961)

ab102961 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (gingiva)
Specification gingiva
Permeabilization No
Fixative Formaldehyde
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Submitted Dec 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"