Anti-Uroplakin Ib antibody (ab102961)

Overview

  • Product name
    Anti-Uroplakin Ib antibody
  • Description
    Rabbit polyclonal to Uroplakin Ib
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 166-196 of Human Uroplakin Ib

  • Positive control
    • K562 cell lysate, Human Brain Tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 30 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions.
  • Tissue specificity
    Bladder epithelium.
  • Sequence similarities
    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications
    N-glycosylated with high-mannose oligosaccharides.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Tetraspanin-20 antibody
    • Tspan-20 antibody
    • TSPAN20 antibody
    • UP1b antibody
    • UPIb antibody
    • UPK1B antibody
    • UPK1B_HUMAN antibody
    • Uroplakin Ib antibody
    • Uroplakin-1b antibody
    see all

Anti-Uroplakin Ib antibody images

  • Anti-Uroplakin Ib antibody (ab102961) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size : 30 kDa
  • Immunohistochemical staining of formalin fixed and paraffin embedded brain tissue followed by HRP-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of K562 cells (green histogram) using ab102961 compared to a negative control cell (blue histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.

References for Anti-Uroplakin Ib antibody (ab102961)

ab102961 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (gingiva)
Specification
gingiva
Permeabilization
No
Fixative
Formaldehyde
Username

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Verified customer

Submitted Dec 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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