Anti-Uroplakin III antibody [SFI-1] (ab78196)

Overview

  • Product nameAnti-Uroplakin III antibody [SFI-1]
    See all Uroplakin III primary antibodies
  • Description
    Mouse monoclonal [SFI-1] to Uroplakin III
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Marmoset (common)
  • Immunogen

    Synthetic peptide corresponding to an internal human Uroplakin III.

  • Positive control
    • Urothelial tissue, bladder.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Proclin
    Constituents: 1% BSA, 0.1% Tween 20, 30mM Tris, 100mM Sodium chloride
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberSFI-1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 21055807
IHC-P 1/20 - 1/30. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate 30 minutes at room temperature.
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.

Target

  • FunctionComponent of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence.
  • Tissue specificityExpressed in ureter.
  • Involvement in diseaseDefects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy.
  • Sequence similaritiesBelongs to the uroplakin-3 family.
  • Cellular localizationEndoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER).
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC119178 antibody
    • OTTHUMP00000028951 antibody
    • UP III antibody
    • UP3a antibody
    • UPIII antibody
    • UPIIIA antibody
    • UPK 3 antibody
    • UPK 3A antibody
    • UPK3 antibody
    • UPK3A antibody
    • UPK3A_HUMAN antibody
    • Uroplakin 3A antibody
    • Uroplakin III antibody
    • Uroplakin-3a antibody
    • Uroplakin3A antibody
    • UroplakinIII antibody
    see all

Anti-Uroplakin III antibody [SFI-1] images

  • ab78196 staining Uroplakin III in mouse bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/600 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    Positivity of only the lumenal cytoplasm of the Umbrella cells is clear.
    As this is a mouse primary antibody, endogenous IgG will also be visualised in the interstitial spaces (red arrowheads), in Plasma cells (black arrowheads) and also in blood vessels if the animal has not been perfused-washed/fixed.

    See Abreview

  • ab78196 staining Uroplakin III in cow ureter tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    Image shows clear, strong labelling of the lumenal membranes of the Umbrella cells.

    See Abreview

  • IHC-P image of Uroplakin-III staining on Marmoset bladder sections using ab78196 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab78196 was diluetd 1:400 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to anti mouse IgG conjugated to biotin (1:200)

     

  • IHC-P image of Uroplakin-III staining on Rat bladder sections using ab78196 (1:400). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab78196 was diluetd 1:1000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Goat polyclonal to anti mouse IgG conjugated to biotin (1:250)

  • ab78196 at 1/20 dilution staining Uroplakin III in human bladder by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

References for Anti-Uroplakin III antibody [SFI-1] (ab78196)

This product has been referenced in:
  • Wu S  et al. Human urine-derived stem cells seeded in a modified 3D porous small intestinal submucosa scaffold for urethral tissue engineering. Biomaterials 32:1317-26 (2011). IHC-Fr ; Human . Read more (PubMed: 21055807) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Goat Tissue sections (Ureter)
Specification Ureter
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jun 02 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Cat Tissue sections (Ureter)
Specification Ureter
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted May 30 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Mouse Tissue sections (Bladder)
Specification Bladder
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 14 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Cow Tissue sections (Ureter)
Specification Ureter
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 14 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Rat Tissue sections (Bladder)
Specification Bladder
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 06 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Marmoset (common) Tissue sections (Bladder)
Specification Bladder
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Dec 05 2013

Thank you for your reply. It is possible that this band may be non-specific, or it may represent Uroplakin III in a different form. Forexample, if the protein is heavily glycosylated it may run higher than predicted. Alternatively, if isoform 2 of Urop...

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Thank you for contacting us. According to the uniprot database (linked below), the predicted molecular weight of the Uroplakin III precursor, isoform 1, is 30.6 kDa. After the removal of the 18 amino acid signal peptide and the additional weight from...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"