Anti-Uroplakin III antibody [SFI-1], prediluted (ab78197)

Overview

  • Product nameAnti-Uroplakin III antibody [SFI-1], prediluted
    See all Uroplakin III primary antibodies
  • Description
    Mouse monoclonal [SFI-1] to Uroplakin III, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to an internal region of Human Uroplakin III.

  • Positive control
    • Urothelial tissue, bladder.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage bufferConstituents: buffer with carrier protein and preservative.
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberSFI-1
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab78197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Ready to use for 30 min at RT. Pre-treatment: Heat Induced Epitope Unmasking in citrate buffer pH 6,0.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence.
    • Tissue specificityExpressed in ureter.
    • Involvement in diseaseDefects in UPK3A are a cause of renal adysplasia (RADYS) [MIM:191830]; also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy.
    • Sequence similaritiesBelongs to the uroplakin-3 family.
    • Cellular localizationEndoplasmic reticulum membrane. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum (ER).
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC119178 antibody
      • OTTHUMP00000028951 antibody
      • UP III antibody
      • UP3a antibody
      • UPIII antibody
      • UPIIIA antibody
      • UPK 3 antibody
      • UPK 3A antibody
      • UPK3 antibody
      • UPK3A antibody
      • UPK3A_HUMAN antibody
      • Uroplakin 3A antibody
      • Uroplakin III antibody
      • Uroplakin-3a antibody
      • Uroplakin3A antibody
      • UroplakinIII antibody
      see all

    Anti-Uroplakin III antibody [SFI-1], prediluted images

    • ab78197 staining Uroplakin III in human bladder by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
    • ab78197 staining Uroplakin III in mouse bladder tissue sections. Tissue was fixed in formaldehyde and heat-mediated antigen retrieval was performed using citric buffer. Blocking was performed with 1% BSA for 10 minutes at 21ºC and ab78197 was used at 1/30 for 2 hours at 21ºC.

      See Abreview

    • ab78197 staining Uroplakin III in rat bladder tissue sections. Tissue was fixed in formaldehyde and heat-mediated antigen retrieval was performed using citric buffer. Blocking was performed with 1% BSA for 10 minutes at 21ºC and ab78197 was used at 1/30 for 2 hours at 21ºC.

      See Abreview

    References for Anti-Uroplakin III antibody [SFI-1], prediluted (ab78197)

    ab78197 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Mouse Tissue sections (Urinary bladder)
    Specification Urinary bladder
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 10 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Rat Tissue sections (Urinary bladder)
    Specification Urinary bladder
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 10 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"