Overview

  • Product nameAnti-URP2 antibody
    See all URP2 primary antibodies
  • Description
    Mouse polyclonal to URP2
  • SpecificityThis antibody is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    URP2 (NP_113659.3, 1 a.a. ~ 663 a.a) full-length human protein.

  • Positive control
    • URP2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). PubMed: 21441448

Target

  • FunctionPlays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs).
    Isoform 2 may act as a repressor of NF-kappa-B and apoptosis.
  • Tissue specificityHighly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies.
  • Involvement in diseaseDefects in FERMT3 are the cause of leukocyte adhesion deficiency type 3 (LAD3) [MIM:612840]; also called leukocyte adhesion deficiency 1 variant (LAD1v). LAD3 is a rare syndrome characterized by infections without pus formation in the presence of a leukocytosis combined with a Glanzmann-type bleeding disorder, resulting from a hematopoietic defect in integrin activation. Symptoms arise from an inability to activate the integrins expressed on hematopoietic cells, including platelets and leukocytes.
  • Sequence similaritiesBelongs to the kindlin family.
    Contains 1 FERM domain.
    Contains 1 PH domain.
  • DomainThe FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
  • Cellular localizationCell projection > podosome. Present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fermitin family homolog 3 antibody
    • Fermitin family member 3 antibody
    • FERMT3 antibody
    • Kind3 antibody
    • Kindlin 3 antibody
    • Kindlin-3 antibody
    • Kindlin3 antibody
    • MGC10966 antibody
    • MIG 2 antibody
    • MIG2 like protein antibody
    • MIG2-like protein antibody
    • MIG2B antibody
    • Unc 112 related protein 2 antibody
    • Unc-112-related protein 2 antibody
    • UNC112C antibody
    • URP2_HUMAN antibody
    • URP2SF antibody
    see all

Anti-URP2 antibody images

  • All lanes : Anti-URP2 antibody (ab67928) at 1/500 dilution

    Lane 1 : URP2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 76 kDa
    Observed band size : 76 kDa
  • Immunofluorescence of ab67928 on HeLa cells [antibody concentration 10 ug/ml].

References for Anti-URP2 antibody (ab67928)

This product has been referenced in:
  • Robert P  et al. A novel leukocyte adhesion deficiency III variant: kindlin-3 deficiency results in integrin- and nonintegrin-related defects in different steps of leukocyte adhesion. J Immunol 186:5273-83 (2011). WB ; Human . Read more (PubMed: 21441448) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelet)
Gel Running Conditions Reduced Denaturing (gradient gel)
Loading amount 20 µg
Specification platelet
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Submitted Jan 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"