Overview

  • Product name
  • Description
    Rabbit polyclonal to URP2
  • Tested applications
    Suitable for: Flow Cyt, ICC, IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human URP2.

    .

  • Positive control
    • This antibody gave a positive signal in the following Tissue Lysates: Rat Kidney, Rat Thymus, Mouse Spleen This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab68040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).

Target

  • Function
    Plays a central role in cell adhesion in hematopoietic cells. Acts by activating the integrin beta-1-3 (ITGB1, ITGB2 and ITGB3). Required for integrin-mediated platelet adhesion and leukocyte adhesion to endothelial cells. Required for activation of integrin beta-2 (ITGB2) in polymorphonuclear granulocytes (PMNs).
    Isoform 2 may act as a repressor of NF-kappa-B and apoptosis.
  • Tissue specificity
    Highly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies.
  • Involvement in disease
    Defects in FERMT3 are the cause of leukocyte adhesion deficiency type 3 (LAD3) [MIM:612840]; also called leukocyte adhesion deficiency 1 variant (LAD1v). LAD3 is a rare syndrome characterized by infections without pus formation in the presence of a leukocytosis combined with a Glanzmann-type bleeding disorder, resulting from a hematopoietic defect in integrin activation. Symptoms arise from an inability to activate the integrins expressed on hematopoietic cells, including platelets and leukocytes.
  • Sequence similarities
    Belongs to the kindlin family.
    Contains 1 FERM domain.
    Contains 1 PH domain.
  • Domain
    The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
  • Cellular localization
    Cell projection > podosome. Present in the F-actin surrounding ring structure of podosomes, which are specialized adhesion structures of hematopoietic cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fermitin family homolog 3 antibody
    • Fermitin family member 3 antibody
    • FERMT3 antibody
    • Kind3 antibody
    • Kindlin 3 antibody
    • Kindlin-3 antibody
    • Kindlin3 antibody
    • MGC10966 antibody
    • MIG 2 antibody
    • MIG2 like protein antibody
    • MIG2-like protein antibody
    • MIG2B antibody
    • Unc 112 related protein 2 antibody
    • Unc-112-related protein 2 antibody
    • UNC112C antibody
    • URP2_HUMAN antibody
    • URP2SF antibody
    see all

Anti-URP2 antibody images

  • All lanes : Anti-URP2 antibody (ab68040) at 1 µg/ml

    Lane 1 : Kidney (Rat) Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate
    Lane 3 : Thymus (Rat) Tissue Lysate

    Lysates/proteins at 10 µg/ml per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 76 kDa
    Observed band size : 76 kDa


    Exposure time : 2 minutes
  • ab68040 used to detect URP2 in Immunocytochemisty/Immunofluorescene in mouse platlets spread on fibrinogen. URPE is co-localized to pseudopodial extensions and near membrane edges. Secondy only (anti-rabbit IgG) negative control was also used.

    See Abreview

  • ab68040 staining URP2 in Human platelets by Flow Cytometry. Platelets were isolated form Platelet rich plasma and suspended in PBS, fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in 2% BSA for 30 minutes. The sample was incubated with the primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
    Gating Strategy: Platelets

    See Abreview

  • IHC image of URP2 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68040, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-URP2 antibody (ab68040)

This product has been referenced in:
  • Singh D  et al. Cooperative Stimulation of Megakaryocytic Differentiation by Gfi1b Gene Targets Kindlin3 and Talin1. PLoS One 11:e0164506 (2016). WB ; Mouse . Read more (PubMed: 27768697) »
  • Canault M  et al. Human CalDAG-GEFI gene (RASGRP2) mutation affects platelet function and causes severe bleeding. J Exp Med 211:1349-62 (2014). Read more (PubMed: 24958846) »

See all 4 Publications for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (platelet)
Specification
platelet
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 17 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cell (RAW264.7)
Specification
RAW264.7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton + 2% BSA in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Platelets)
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 30 min
Gating Strategy
Platelets
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 03 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Meg01-Human megakaryoblast)
Specification
Meg01-Human megakaryoblast
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (platelet)
Specification
platelet
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (CHO transfected with human protein)
Loading amount
40 µg
Specification
CHO transfected with human protein
Gel Running Conditions
Reduced Denaturing (7.5 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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