The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 37.2 kDa). For optimal results, primary antibody incubations should be performed at 4°C.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this.
Detection of USF1 by Western blot analysis. The presence of USF1 in nuclear extracts derived from the Jurkat cell line is confirmed using ab12128 at a 1:250 dilution (lane 1). As demonstrated in lane 2, the signal corresponding to USF1 is eliminated by incubating the reaction mixture together with the immunizing peptide.