Overview

  • Product name
    Anti-USF1 antibody [EPR6430]
    See all USF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6430] to USF1
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • K-562, Jurkat and HeLa cell lysates; Jurkat cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab125020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Relevance
      USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • bHLHb11 antibody
      • Class B basic helix-loop-helix protein 11 antibody
      • FCHL 1 antibody
      • FCHL antibody
      • FCHL1 antibody
      • HGNC:12593 antibody
      • HYPLIP 1 antibody
      • HYPLIP1 antibody
      • Major late transcription factor 1 antibody
      • Major late transcription factor antibody
      • MLTF antibody
      • MLTF I antibody
      • MLTFI antibody
      • Transcription factor USF-1 antibody
      • UEF antibody
      • Upstream stimulatory factor 1 antibody
      • Upstream transcription factor 1 antibody
      • Upstream transcription factor 1, isoform CRA_a antibody
      • USF 1 antibody
      • USF antibody
      see all

    Images

    • All lanes : Anti-USF1 antibody [EPR6430] (ab125020) at 1/10000 dilution

      Lane 1 : K-562 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Hela cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 34 kDa
    • Flow cytometric analysis of permeabilized Jurkat cells using ab125020 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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