Class B basic helix-loop-helix protein 12 antibody
FOS interacting protein antibody
FOS-interacting protein antibody
Major late transcription factor 2 antibody
Upstream stimulatory factor 2 antibody
Upstream transcription factor 2 antibody
USF 2 antibody
Anti-USF2 antibody images
Flow Cytometry - Anti-USF2 antibody (ab60931)
Overlay histogram showing HeLa cells stained with ab60931 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60931, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - USF2 antibody (ab60931)
All lanes : Anti-USF2 antibody (ab60931)
Lane 1 : HeLa cell lysate Lane 2 : A431 cell lysate Lane 3 : Jurkat cell lysate Lane 4 : MCF7 cell lysate Lane 5 : K562 cell lysate Lane 6 : HepG2 cell lysate Lane 7 : HeLa nuclear extract Lane 8 : RAW264.7 cell lysate Lane 9 : PC12 cell lysate Lane 10 : NIH 3T3 cell lysate Lane 11 : IMR32 cell lysate
Lysates/proteins at 50 µg per lane.
Secondary Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution