Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Class B basic helix-loop-helix protein 12 antibody
FOS interacting protein antibody
FOS-interacting protein antibody
Major late transcription factor 2 antibody
Upstream stimulatory factor 2 antibody
Upstream transcription factor 2 antibody
USF 2 antibody
Anti-USF2 antibody images
Flow Cytometry - Anti-USF2 antibody (ab60931)
Overlay histogram showing HeLa cells stained with ab60931 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60931, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - USF2 antibody (ab60931)
All lanes : Anti-USF2 antibody (ab60931)
Lane 1 : HeLa cell lysate Lane 2 : A431 cell lysate Lane 3 : Jurkat cell lysate Lane 4 : MCF7 cell lysate Lane 5 : K562 cell lysate Lane 6 : HepG2 cell lysate Lane 7 : HeLa nuclear extract Lane 8 : RAW264.7 cell lysate Lane 9 : PC12 cell lysate Lane 10 : NIH 3T3 cell lysate Lane 11 : IMR32 cell lysate
Lysates/proteins at 50 µg per lane.
Secondary Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution