Overview

  • Product nameAnti-USP1 antibody
    See all USP1 primary antibodies
  • Description
    Rabbit polyclonal to USP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 701-750 (SETSDTTGTH ESDRNKESSD QTGINISGFE NKISYVVQSL KEYEGKWLLF) of Human USP1 (NP_003359).

  • Positive control
    • Human fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab108104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 88 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionNegative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
  • Sequence similaritiesBelongs to the peptidase C19 family.
  • Developmental stageCell cycle-regulated. Highest level during S phase.
  • Post-translational
    modifications
    Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
    Ubiquitinated; leading to its subsequent proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 1 antibody
    • hUBP antibody
    • Ubiquitin carboxyl terminal hydrolase 1 antibody
    • Ubiquitin carboxyl-terminal hydrolase 1 antibody
    • Ubiquitin specific peptidase 1 antibody
    • Ubiquitin specific protease 1 antibody
    • Ubiquitin thioesterase 1 antibody
    • Ubiquitin thiolesterase 1 antibody
    • Ubiquitin-specific-processing protease 1 antibody
    • UBP antibody
    • UBP1_HUMAN antibody
    • USP 1 antibody
    • Usp1 antibody
    see all

Anti-USP1 antibody images



  • Predicted band size : 88 kDa
    Western blot analysis of 293T cell lysate labeling USP1 with ab108104 at 1.0µg/ml.
  • Anti-USP1 antibody (ab108104) at 1 µg/ml + Human fetal brain lysate at 10 µg

    Predicted band size : 88 kDa
    Gel concentration: 12%.

References for Anti-USP1 antibody (ab108104)

This product has been referenced in:
  • Yoon JH  et al. PAR-CLIP analysis uncovers AUF1 impact on target RNA fate and genome integrity. Nat Commun 5:5248 (2014). WB ; Human . Read more (PubMed: 25366541) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (cultured primary NK cells)
Gel Running Conditions Reduced Denaturing (4-12% bis-tris, MES buffers)
Loading amount 800000 cells
Treatment expanded in IL15
Specification cultured primary NK cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Lynn Corcoran

Verified customer

Submitted Jun 23 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells)
Loading amount 20 µg
Specification HEK293 cells
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"