Application notesThis antibody gave a positive result in ELISA against the immunizing peptide (ab28510).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionNegative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
Sequence similaritiesBelongs to the peptidase C19 family.
Developmental stageCell cycle-regulated. Highest level during S phase.
Post-translational modificationsAutocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis. Ubiquitinated; leading to its subsequent proteasomal degradation. Phosphorylated upon DNA damage, probably by ATM or ATR.
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - Anti-USP1 antibody (ab27868)Image from Garcia-Santisteban I et al., PLoS One. 2012;7(6):e38570. Epub 2012 Jun 6. Fig 1.; doi:10.1371/journal.pone.0038570; June 6, 2012, PLoS ONE 7(6): e38570.
Immunofluorescence analysis of HEK293T cells, staining USP1 with ab27868 at 1/200 dilution.
References for Anti-USP1 antibody (ab27868)
This product has been referenced in:
Garcia-Santisteban I et al. Two Nuclear Localization Signals in USP1 Mediate Nuclear Import of the USP1/UAF1 Complex. PLoS One7:e38570 (2012).
Read more (PubMed: 22701671) »