Overview

  • Product name
    Anti-USP1 antibody - C-terminal
    See all USP1 primary antibodies
  • Description
    Rabbit polyclonal to USP1 - C-terminal
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human USP1 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: O94782

  • Positive control
    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab227551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 88 kDa.

Target

  • Function
    Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.
  • Sequence similarities
    Belongs to the peptidase C19 family.
  • Developmental stage
    Cell cycle-regulated. Highest level during S phase.
  • Post-translational
    modifications
    Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.
    Ubiquitinated; leading to its subsequent proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 1 antibody
    • hUBP antibody
    • Ubiquitin carboxyl terminal hydrolase 1 antibody
    • Ubiquitin carboxyl-terminal hydrolase 1 antibody
    • Ubiquitin specific peptidase 1 antibody
    • Ubiquitin specific protease 1 antibody
    • Ubiquitin thioesterase 1 antibody
    • Ubiquitin thiolesterase 1 antibody
    • Ubiquitin-specific-processing protease 1 antibody
    • UBP antibody
    • UBP1_HUMAN antibody
    • USP 1 antibody
    • Usp1 antibody
    see all

Images

  • All lanes : Anti-USP1 antibody - C-terminal (ab227551) at 1/3000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 88 kDa

    5% SDS-PAGE gel.

References

ab227551 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227551.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up