Overview

  • Product nameAnti-USP10 antibody [2E1]
    See all USP10 primary antibodies
  • Description
    Mouse monoclonal [2E1] to USP10
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human USP10 produced in HEK293T cells (NP_005144).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY USP10 cDNA; HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cell extracts; Human breast, pancreas, endometrium, endometrium adenocarcinoma, prostate, bladder carcinoma, lymphoma and tonsil tissues; COS7 cells transiently transfected with pCMV6-ENTRY USP10; Hela cells
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab119418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 87 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionHydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the peptidase C19 family. USP10 subfamily.
  • Post-translational
    modifications
    Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 10 antibody
    • KIAA0190 antibody
    • MGC124997 antibody
    • MGC2621 antibody
    • Ubiquitin carboxyl terminal hydrolase 10 antibody
    • Ubiquitin carboxyl-terminal hydrolase 10 antibody
    • Ubiquitin specific peptidase 10 antibody
    • Ubiquitin specific processing protease 10 antibody
    • Ubiquitin specific protease 10 antibody
    • Ubiquitin thioesterase 10 antibody
    • Ubiquitin thiolesterase 10 antibody
    • Ubiquitin-specific-processing protease 10 antibody
    • UBP10_HUMAN antibody
    • UBPO antibody
    • USP 10 antibody
    • USP10 antibody
    see all

Anti-USP10 antibody [2E1] images

  • All lanes : Anti-USP10 antibody [2E1] (ab119418) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY USP10 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 87 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200835)
  • All lanes : Anti-USP10 antibody [2E1] (ab119418) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 87 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200835)
  • ab119418, at 1/150 dilution staining USP10 in paraffin-embedded Human breast tissue by Immunohistochemistry.
  • ab119418, at 1/150 dilution staining USP10 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • ab119418, at 1/150 dilution staining USP10 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab119418, at 1/150 dilution staining USP10 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab119418, at 1/150 dilution staining USP10 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab119418, at 1/150 dilution staining USP10 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab119418, at 1/150 dilution staining USP10 in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.
  • ab119418, at 1/150 dilution staining USP10 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
  • ab119418, at 1/100 dilution, staining USP10 in COS7 cells transiently transfected with pCMV6-ENTRY USP10 by Immunofluorescence.
  • Flow cytometric analysis of USP10 in Hela cells using ab119418 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).

References for Anti-USP10 antibody [2E1] (ab119418)

ab119418 has not yet been referenced specifically in any publications.

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