Overview

  • Product name
  • Description
    Rabbit polyclonal to USP10
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant GST fusion protein (mouse Usp10). This antibody was generated against amino acids 1-300 of mouse USP10.

Properties

Applications

Our Abpromise guarantee covers the use of ab72486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ICC/IF
IP
  • Application notes
    ICC/IF: 1/200.
    IHC-P: Use at an assay dependent dilution (PMID 20096447).
    IP: 1/500.
    WB: 1/1000. Predicted molecular weight: 87 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the peptidase C19 family. USP10 subfamily.
    • Post-translational
      modifications
      Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
    • Cellular localization
      Cytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 10 antibody
      • KIAA0190 antibody
      • MGC124997 antibody
      • MGC2621 antibody
      • Ubiquitin carboxyl terminal hydrolase 10 antibody
      • Ubiquitin carboxyl-terminal hydrolase 10 antibody
      • Ubiquitin specific peptidase 10 antibody
      • Ubiquitin specific processing protease 10 antibody
      • Ubiquitin specific protease 10 antibody
      • Ubiquitin thioesterase 10 antibody
      • Ubiquitin thiolesterase 10 antibody
      • Ubiquitin-specific-processing protease 10 antibody
      • UBP10_HUMAN antibody
      • UBPO antibody
      • USP 10 antibody
      • USP10 antibody
      see all

    Images

    References

    This product has been referenced in:
    • Li L  et al. A specific E3 ligase/deubiquitinase pair modulates TBP protein levels during muscle differentiation. Elife 4:e08536 (2015). Read more (PubMed: 26393420) »
    • Xia HG  et al. Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death. J Cell Biol 210:705-16 (2015). Read more (PubMed: 26323688) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Methanol
    Permeabilization
    No
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Feb 01 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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