Validated using a knockout cell line

Anti-USP10 antibody [EPR4261] (ab109219)


  • Product name
    Anti-USP10 antibody [EPR4261]
    See all USP10 primary antibodies
  • Description
    Rabbit monoclonal [EPR4261] to USP10
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human USP10.

  • Positive control
    • WB: HeLa, 293T, A375 and A549 cell lysates IHC-P: Human colon tissue This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab109219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 87 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the peptidase C19 family. USP10 subfamily.
    • Post-translational
      Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
    • Cellular localization
      Cytoplasm. Nucleus. Early endosome. Cytoplasmic in normal conditions. After DNA damage, translocates to the nucleus following phosphorylation by ATM.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 10 antibody
      • KIAA0190 antibody
      • MGC124997 antibody
      • MGC2621 antibody
      • Ubiquitin carboxyl terminal hydrolase 10 antibody
      • Ubiquitin carboxyl-terminal hydrolase 10 antibody
      • Ubiquitin specific peptidase 10 antibody
      • Ubiquitin specific processing protease 10 antibody
      • Ubiquitin specific protease 10 antibody
      • Ubiquitin thioesterase 10 antibody
      • Ubiquitin thiolesterase 10 antibody
      • Ubiquitin-specific-processing protease 10 antibody
      • UBP10_HUMAN antibody
      • UBPO antibody
      • USP 10 antibody
      • USP10 antibody
      see all

    Anti-USP10 antibody [EPR4261] images

    • Predicted band size : 87 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: USP10 knockout HAP1 cell lysate (20 µg)
      Lane 3: A549 cell lysate (20 µg)
      Lane 4: A375 cell lysate (20 µg)
      Lanes 1 to 4: Merged signal (red and green). Green - ab109219 observed at 115 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab109219 was shown to recognize USP10 when USP10 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP10 knockout samples were subjected to SDS-PAGE. ab109219 and ab8245 (loading control to GAPDH) were both diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-USP10 antibody [EPR4261] (ab109219) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A375 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 87 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • ab109219, at a 1/250 dilution, staining USP10 in paraffin embedded Human colon tissue by Immunohistochemistry.
    • ICC/IF image of ab109219 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109219 at 1/50 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    References for Anti-USP10 antibody [EPR4261] (ab109219)

    ab109219 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (EC109)
    Gel Running Conditions
    Non-reduced Denaturing (10%SDS)
    Loading amount
    80 µg
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

    Qingyuan Yang

    Verified customer

    Submitted Jan 08 2016


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