Overview

  • Product nameAnti-USP12 antibody
    See all USP12 primary antibodies
  • Description
    Rabbit polyclonal to USP12
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (TRLRKENELF DNYMQQDAHE FLNYLLNTIA DILQEERKQE KQNGRLPNGN) of Human USP12 (NP_872294).

  • Positive control
    • Human Placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionDeubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.
  • Sequence similaritiesBelongs to the peptidase C19 family. USP12/USP46 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 12 antibody
    • OTTHUMP00000042337 antibody
    • UBH 1 antibody
    • UBH1 antibody
    • Ubiquitin carboxyl terminal hydrolase 12 antibody
    • Ubiquitin carboxyl-terminal hydrolase 12 antibody
    • Ubiquitin hydrolyzing enzyme 1 antibody
    • Ubiquitin specific peptidase 12 antibody
    • Ubiquitin specific processing protease 12 antibody
    • Ubiquitin specific protease 12 like 1 antibody
    • Ubiquitin thioesterase 12 antibody
    • Ubiquitin thiolesterase 12 antibody
    • Ubiquitin-hydrolyzing enzyme 1 antibody
    • Ubiquitin-specific-processing protease 12 antibody
    • UBP12_HUMAN antibody
    • USP 12 antibody
    • Usp12 antibody
    • USP12L1 antibody
    see all

Anti-USP12 antibody images

  • Anti-USP12 antibody (ab89870) at 1 µg/ml (in 5% skim milk / PBS buffer) + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa

References for Anti-USP12 antibody (ab89870)

ab89870 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Xenopus laevis Cell lysate - whole cell (Egg extracts)
Specification Egg extracts
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

Submitted Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"