Overview

  • Product name
  • Description
    Rabbit polyclonal to USP12
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (TRLRKENELF DNYMQQDAHE FLNYLLNTIA DILQEERKQE KQNGRLPNGN) of Human USP12 (NP_872294).

  • Positive control
    • Human Placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab89870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.
  • Sequence similarities
    Belongs to the peptidase C19 family. USP12/USP46 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 12 antibody
    • OTTHUMP00000042337 antibody
    • UBH 1 antibody
    • UBH1 antibody
    • Ubiquitin carboxyl terminal hydrolase 12 antibody
    • Ubiquitin carboxyl-terminal hydrolase 12 antibody
    • Ubiquitin hydrolyzing enzyme 1 antibody
    • Ubiquitin specific peptidase 12 antibody
    • Ubiquitin specific processing protease 12 antibody
    • Ubiquitin specific protease 12 like 1 antibody
    • Ubiquitin thioesterase 12 antibody
    • Ubiquitin thiolesterase 12 antibody
    • Ubiquitin-hydrolyzing enzyme 1 antibody
    • Ubiquitin-specific-processing protease 12 antibody
    • UBP12_HUMAN antibody
    • USP 12 antibody
    • Usp12 antibody
    • USP12L1 antibody
    see all

Images

  • Anti-USP12 antibody (ab89870) at 1 µg/ml (in 5% skim milk / PBS buffer) + Placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 43 kDa



    Gel concentration: 12%

References

ab89870 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - whole cell (Egg extracts)
Specification
Egg extracts
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

Submitted Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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