Overview

  • Product nameAnti-USP13 antibody
    See all USP13 primary antibodies
  • Description
    Rabbit polyclonal to USP13
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human USP13 (NP_003931.2).

  • Positive control
    • HeLa and 293T whole cell lysate This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab99421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Tissue specificityHighly expressed in ovary and testes.
  • Sequence similaritiesBelongs to the peptidase C19 family.
    Contains 2 UBA domains.
    Contains 1 UBP-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 13 antibody
    • Isopeptidase T 3 antibody
    • Isopeptidase T-3 antibody
    • Isopeptidase T3 antibody
    • ISOT 3 antibody
    • ISOT-3 antibody
    • ISOT3 antibody
    • Ubiquitin carboxyl terminal hydrolase 13 antibody
    • Ubiquitin carboxyl-terminal hydrolase 13 antibody
    • Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
    • Ubiquitin specific peptidase 13 antibody
    • Ubiquitin specific processing protease 13 antibody
    • Ubiquitin specific protease 13 antibody
    • Ubiquitin thioesterase 13 antibody
    • Ubiquitin thiolesterase 13 antibody
    • Ubiquitin-specific-processing protease 13 antibody
    • UBP13 antibody
    • UBP13_HUMAN antibody
    • USP 13 antibody
    • USP13 antibody
    see all

Anti-USP13 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma tissues labelling USP13 with ab99421 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
  • ICC/IF image of ab99421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99421 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-USP13 antibody (ab99421) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 97 kDa


    Exposure time : 3 minutes
  • ab99421 at 1 µg/ml detecting USP13 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 10 second exposure.
    For immunoprecipitation, ab99421 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99421
    Lane 2; IP using ab99423, a rabbit anti-USP13 antibody which recognizes a downstream epitope.
    Lane 3; IP using control IgG.

References for Anti-USP13 antibody (ab99421)

This product has been referenced in:
  • Ouyang H  et al. Protein Aggregates Are Recruited to Aggresome by Histone Deacetylase 6 via Unanchored Ubiquitin C Termini. J Biol Chem 287:2317-27 (2012). Read more (PubMed: 22069321) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab99421.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"