• Product nameAnti-USP13 antibody
    See all USP13 primary antibodies
  • Description
    Rabbit polyclonal to USP13
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 750-800 of Human USP13 (NP_003931.2).

  • Positive control
    • HeLa and 293T whole cell lysates.



Our Abpromise guarantee covers the use of ab99423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Tissue specificityHighly expressed in ovary and testes.
  • Sequence similaritiesBelongs to the peptidase C19 family.
    Contains 2 UBA domains.
    Contains 1 UBP-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 13 antibody
    • Isopeptidase T 3 antibody
    • Isopeptidase T-3 antibody
    • Isopeptidase T3 antibody
    • ISOT 3 antibody
    • ISOT-3 antibody
    • ISOT3 antibody
    • Ubiquitin carboxyl terminal hydrolase 13 antibody
    • Ubiquitin carboxyl-terminal hydrolase 13 antibody
    • Ubiquitin specific peptidase 13 (isopeptidase T3) antibody
    • Ubiquitin specific peptidase 13 antibody
    • Ubiquitin specific processing protease 13 antibody
    • Ubiquitin specific protease 13 antibody
    • Ubiquitin thioesterase 13 antibody
    • Ubiquitin thiolesterase 13 antibody
    • Ubiquitin-specific-processing protease 13 antibody
    • UBP13 antibody
    • UBP13_HUMAN antibody
    • USP 13 antibody
    • USP13 antibody
    see all

Anti-USP13 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling USP13 with ab99423 at 1/5000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-USP13 antibody (ab99423) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 97 kDa

    Exposure time : 30 seconds
  • ab99423 at 1 µg/ml detecting USP13 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 10 second exposure.
    For immunoprecipitation, ab99423 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99421, a rabbit anti-USP13 antibody which recognizes an upstream epitope.
    Lane 2; IP using ab99423.
    Lane 3; IP using control IgG.

References for Anti-USP13 antibody (ab99423)

ab99423 has not yet been referenced specifically in any publications.

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