The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
Use a concentration of 2.5 - 3.8 µg/ml.
Perform heat mediated antigen retrieval with citrate buffer (pH6).
FunctionProtease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
Tissue specificityExpressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
Sequence similaritiesBelongs to the peptidase C19 family. Contains 1 DUSP domain.
Post-translational modificationsPhosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome. Ubiquitinated, leading to degradation by the proteasome.