1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
FunctionProtease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
Tissue specificityExpressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
Sequence similaritiesBelongs to the peptidase C19 family. Contains 1 DUSP domain.
Post-translational modificationsPhosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome. Ubiquitinated, leading to degradation by the proteasome.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling USP15 with ab71713 at 1/200 (1µg/ml). Detection: DAB.
Western blot - USP15 antibody (ab71713)
All lanes : Anti-USP15 antibody (ab71713) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size : 112 kDa Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Western blot - Anti-USP15 antibody (ab71713)
All lanes : Anti-USP15 antibody (ab71713) at 1 µg/ml
Lane 1 : NIH3T3 whole cell lysate Lane 2 : TCMK-1 whole cell lysate Lane 3 : 4T1 whole cell lysate Lane 4 : CT26.WT whole cell lysate Lane 5 : C6 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique
Predicted band size : 112 kDa
Exposure time : 3 minutes
Immunoprecipitation - USP15 antibody (ab71713)
USP15 antibody (ab71713) recognizing human USP15 in HeLa whole cell lysate (1 mg, 20% of IP loaded). Lane 1: USP15 antibody (ab71713) blotted at 1.0 µg/ml and used for IP at 3 µg/mg lysate.
Lane 2: Control IgG Detection by chemiluminescence with exposure time of 3 minutes.
References for Anti-USP15 antibody (ab71713)
has not yet been referenced specifically in any publications.
Publishing research using ab71713? Please let us know so that we can cite the reference in this datasheet.
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