Anti-USP15 antibody (ab97533)
Key features and details
- Rabbit polyclonal to USP15
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP15 antibody
See all USP15 primary antibodies -
Description
Rabbit polyclonal to USP15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within amino acids 941-952 of Human USP15 (NP_006304).
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Positive control
- WB: 293T, A431, H1299, HeLa, MOLT4 and Raji cell lines IHC-P: N87 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 112 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 112 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. -
Tissue specificity
Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 DUSP domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome.
Ubiquitinated, leading to degradation by the proteasome. - Information by UniProt
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Database links
- Entrez Gene: 9958 Human
- Omim: 604731 Human
- SwissProt: Q9Y4E8 Human
- Unigene: 434951 Human
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Alternative names
- Deubiquitinating enzyme 15 antibody
- Deubiquitinating enzyme antibody
- EC 3 1 2 15 antibody
see all
Images
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Anti-USP15 antibody (ab97533) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 112 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A431, using USP15(GTX104644) antibody at 1:200 dilution.
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Immunohistochemical analysis of USP15 in paraffin embedded N87 xenograft, using ab97533 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab97533 has been referenced in 4 publications.
- Kim MJ et al. USP15 negatively regulates lung cancer progression through the TRAF6-BECN1 signaling axis for autophagy induction. Cell Death Dis 13:348 (2022). PubMed: 35422093
- Yang H et al. GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A. iScience 25:104952 (2022). PubMed: 36065190
- Teyra J et al. Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15. Structure 27:590-605.e5 (2019). PubMed: 30713027
- Eichhorn PJ et al. USP15 stabilizes TGF-ß receptor I and promotes oncogenesis through the activation of TGF-ß signaling in glioblastoma. Nat Med 18:429-35 (2012). PubMed: 22344298