The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 175 kDa (predicted molecular weight: 145 kDa).
FunctionDeubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates.
Sequence similaritiesBelongs to the peptidase C19 family. Contains 2 CS domains. Contains 1 MYND-type zinc finger.
Post-translational modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.