Overview

  • Product name
  • Description
    Rabbit polyclonal to USP20
  • Host species
    Rabbit
  • Specificity
    BLAST analysis of the peptide immunogen showed no homology with other human proteins.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Opossum, Common marmoset, Orangutan, Bat, Platypus, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human USP20 (internal sequence). Synthetic peptide corresponding to 18 amino acids within USP20 (Q9Y2K6, NM_006676, NP_006667.2).

  • Positive control
    • Human adrenal medulla tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab150741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Sequence similarities
    Belongs to the peptidase C19 family. USP20/USP33 subfamily.
    Contains 2 DUSP domains.
    Contains 1 UBP-type zinc finger.
  • Domain
    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue.
  • Post-translational
    modifications
    Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
  • Cellular localization
    Cytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 20 antibody
    • hVDU2 antibody
    • KIAA1003 antibody
    • LSFR 3A antibody
    • LSFR3A antibody
    • pVHL interacting deubiquitinating enzyme 2 antibody
    • Ubiquitin carboxyl terminal hydrolase 20 antibody
    • Ubiquitin carboxyl-terminal hydrolase 20 antibody
    • ubiquitin specific peptidase 20 antibody
    • Ubiquitin specific processing protease 20 antibody
    • Ubiquitin specific protease 20 antibody
    • Ubiquitin thioesterase 20 antibody
    • Ubiquitin thiolesterase 20 antibody
    • Ubiquitin-specific-processing protease 20 antibody
    • UBP20_HUMAN antibody
    • USP 20 antibody
    • USP20 antibody
    • VDU 2 antibody
    • VDU2 antibody
    • VHL interacting deubiquitinating enzyme 2 antibody
    • VHL-interacting deubiquitinating enzyme 2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal medulla tissue labelled with ab150741 at 10 µg/ml.

References

ab150741 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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