Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab5272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Use at a concentration of 3 µg/ml.
    Peptide ELISA: antibody detection limit dilution 1/3200
    Western Blot: Preliminary experiments gave no signal but low background in Human Liver extracts at up to 1µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDeubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • Sequence similaritiesBelongs to the peptidase C19 family. USP20/USP33 subfamily.
      Contains 2 DUSP domains.
      Contains 1 UBP-type zinc finger.
    • DomainThe UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue.
    • Post-translational
      modifications
      Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
    • Cellular localizationCytoplasm > perinuclear region. According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 20 antibody
      • hVDU2 antibody
      • KIAA1003 antibody
      • LSFR 3A antibody
      • LSFR3A antibody
      • pVHL interacting deubiquitinating enzyme 2 antibody
      • Ubiquitin carboxyl terminal hydrolase 20 antibody
      • Ubiquitin carboxyl-terminal hydrolase 20 antibody
      • ubiquitin specific peptidase 20 antibody
      • Ubiquitin specific processing protease 20 antibody
      • Ubiquitin specific protease 20 antibody
      • Ubiquitin thioesterase 20 antibody
      • Ubiquitin thiolesterase 20 antibody
      • Ubiquitin-specific-processing protease 20 antibody
      • UBP20_HUMAN antibody
      • USP 20 antibody
      • USP20 antibody
      • VDU 2 antibody
      • VDU2 antibody
      • VHL interacting deubiquitinating enzyme 2 antibody
      • VHL-interacting deubiquitinating enzyme 2 antibody
      see all

    Anti-USP20 antibody images

    • ab5272 at 3ug/ml staining USP20 in human lymph node tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave in Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

    References for Anti-USP20 antibody (ab5272)

    ab5272 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"