Validated using a knockout cell line
Recombinant
RabMAb

Anti-USP22 antibody [EPR4352(2)] (ab109435)

Overview

  • Product name
    Anti-USP22 antibody [EPR4352(2)]
    See all USP22 primary antibodies
  • Description
    Rabbit monoclonal [EPR4352(2)] to USP22
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in human USP22

  • Positive control
    • HeLa, HAP1, human placenta and HT-1376 lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000 - 1/5000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
    • Tissue specificity
      Moderately expressed in various tissues including heart and skeletal muscle, and weakly expressed in lung and liver.
    • Sequence similarities
      Belongs to the peptidase C19 family. UBP8 subfamily.
      Contains 1 UBP-type zinc finger.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Deubiquitinating enzyme 22 antibody
      • KIAA1063 antibody
      • Ubiquitin carboxyl terminal hydrolase 22 antibody
      • Ubiquitin carboxyl-terminal hydrolase 22 antibody
      • Ubiquitin specific peptidase 22 antibody
      • Ubiquitin specific peptidase 3 like antibody
      • Ubiquitin specific processing protease 22 antibody
      • Ubiquitin specific protease 22 antibody
      • Ubiquitin thioesterase 22 antibody
      • Ubiquitin thiolesterase 22 antibody
      • Ubiquitin-specific-processing protease 22 antibody
      • UBP22_HUMAN antibody
      • USP 22 antibody
      • Usp22 antibody
      • USP3L antibody
      see all

    Anti-USP22 antibody [EPR4352(2)] images



    • Predicted band size : 60 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: USP22 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109435 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.


      Ab109435 was shown to specifically react with USP22 in wild-type cells along with additional cross-reactive bands. The band was not seen in USP22 knockout HAP1 cells. Wild-type and USP22 knockout samples were subjected to SDS-PAGE. ab109435 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-USP22 antibody [EPR4352(2)] (ab109435) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Human placenta lysate
      Lane 3 : HT-1376 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 60 kDa
    • Flow cytometric analysis of permeabilized HeLa cells using 1/10 ab109435 (red) or a rabbit IgG (negative) (green).
    • ab109435 (1/200) staining USP22 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-USP22 antibody [EPR4352(2)] (ab109435)

    ab109435 has not yet been referenced specifically in any publications.

    Product Wall

    As requested, I have issued free of charge replacements.


    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also cover...

    Read More

    Thank you for following up your earlier complaints about these two antibodies, ab28664 and ab109435, with more details of your protocols and samples.

    I will be happy to replace them. We have not received other complaints about them but I sug...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Sep 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up