Anti-USP24 antibody (ab72241)
Key features and details
- Rabbit polyclonal to USP24
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-USP24 antibody
See all USP24 primary antibodies -
Description
Rabbit polyclonal to USP24 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide from a region between residue 2410 and the C-terminus (residue 2460) of human USP24 (XP_371254.5)
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Positive control
- Whole cell lysate from Hela and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IP |
Notes |
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WB: 1/2,000 - 1/10,000. Detects bands of approximately 71 kDa and 250 kDa (predicted molecular weight: 211 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Involved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome. -
Sequence similarities
Belongs to the peptidase C19 family.
Contains 1 UBA domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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Database links
- Entrez Gene: 456881 Chimpanzee
- Entrez Gene: 479558 Dog
- Entrez Gene: 23358 Human
- Entrez Gene: 329908 Mouse
- Entrez Gene: 313427 Rat
- Entrez Gene: 717178 Rhesus monkey
- Omim: 610569 Human
- SwissProt: Q9UPU5 Human
see all -
Alternative names
- Deubiquitinating enzyme 24 antibody
- KIAA1057 antibody
- Ubiquitin carboxyl terminal hydrolase 24 antibody
see all
Images
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All lanes : Anti-USP24 antibody (ab72241) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 211 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation/ Western Blot of USP24.
Lane 1: ab72241 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72241 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab72241 has been referenced in 3 publications.
- Alzhanova D et al. Novel modulators of p53-signaling encoded by unknown genes of emerging viruses. PLoS Pathog 17:e1009033 (2021). PubMed: 33411764
- Luo H et al. WP1130 reveals USP24 as a novel target in T-cell acute lymphoblastic leukemia. Cancer Cell Int 19:56 (2019). PubMed: 30911287
- Wang K et al. Transcriptional regulation of human USP24 gene expression by NF-kappa B. J Neurochem 128:818-28 (2014). PubMed: 24286619