Anti-USP25 antibody [1277CT376106171] (ab188362)

Overview

  • Product name
    Anti-USP25 antibody [1277CT376106171]
    See all USP25 primary antibodies
  • Description
    Mouse monoclonal [1277CT376106171] to USP25
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human USP25. The exact sequence is proprietary.
    Database link: Q9UHP3

  • Positive control
    • Daudi cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab188362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 122 kDa.

Target

  • Function
    Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
    The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
  • Tissue specificity
    Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
  • Sequence similarities
    Belongs to the peptidase C19 family.
    Contains 1 UBA-like domain.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • Post-translational
    modifications
    Acetylated.
    Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
    Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
    Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
  • Cellular localization
    Cytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 25 antibody
    • Ubiquitin carboxyl-terminal hydrolase 25 antibody
    • Ubiquitin specific peptidase 25 antibody
    • ubiquitin specific protease 25 antibody
    • ubiquitin specific protease USP25 antibody
    • Ubiquitin thiolesterase 25 antibody
    • Ubiquitin-specific-processing protease 25 antibody
    • UBP25_HUMAN antibody
    • USP on chromosome 21 antibody
    • USP21 antibody
    • USP25 antibody
    see all

Images

  • Anti-USP25 antibody [1277CT376106171] (ab188362) at 1/1000 dilution + Daudi cell lysate at 35 µg

    Secondary
    Goat anti-Mouse IgG H&L(HRP) at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 122 kDa

References

ab188362 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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