• Product name
  • Description
    Rabbit polyclonal to USP25
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human USP25 aa 1037-1068 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9UHP3

  • Positive control
    • WB: Rat testis tissue lysate and HeLa cell lysate. IHC-P: Human breast carcinoma tissue.



Our Abpromise guarantee covers the use of ab38498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa).
IHC-P 1/50 - 1/100.


  • Function
    Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
    The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
  • Tissue specificity
    Isoform USB25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USB25b is found in all tissues except heart and skeletal muscle. Isoform USB25m is heart and skeletal muscle specific.
  • Sequence similarities
    Belongs to the peptidase C19 family.
    Contains 1 UBA-like domain.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
  • Post-translational
    Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially by SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
    Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
    Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
  • Cellular localization
    Cytoplasm and Cytoplasm. Nucleus. Some transient punctuate nuclear location in myotubes during myocyte development.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 25 antibody
    • Ubiquitin carboxyl-terminal hydrolase 25 antibody
    • Ubiquitin specific peptidase 25 antibody
    • ubiquitin specific protease 25 antibody
    • ubiquitin specific protease USP25 antibody
    • Ubiquitin thiolesterase 25 antibody
    • Ubiquitin-specific-processing protease 25 antibody
    • UBP25_HUMAN antibody
    • USP on chromosome 21 antibody
    • USP21 antibody
    • USP25 antibody
    see all

Anti-USP25 antibody images

  • All lanes : Anti-USP25 antibody (ab38498)

    Lane 1 : Rat testis tissue lysate
    Lane 2 : USP25-transfected HeLa cell lysates

    Predicted band size : 125 kDa
    Observed band size : 125 kDa
    Transfection data is kindly provided by Dr. B. Pierrat from the Novartis Institute for Biomedical Research (Basel, Switzerland).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling USP25 with ab38498. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

References for Anti-USP25 antibody (ab38498)

ab38498 has not yet been referenced specifically in any publications.

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