Overview

  • Product nameAnti-USP26 antibody
    See all USP26 primary antibodies
  • Description
    Rabbit polyclonal to USP26
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human USP26.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HeLa Nuclear; HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab101650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 24922532
IHC-Fr Use at an assay dependent concentration. PubMed: 24922532
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 104 kDa).

Target

  • FunctionInvolved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome (By similarity). Deubiquinates the androgen receptor and regulates the androgen receptor signaling pathway.
  • Sequence similaritiesBelongs to the peptidase C19 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 26 antibody
    • MGC120066 antibody
    • MGC120067 antibody
    • MGC120068 antibody
    • Ubiquitin carboxyl terminal hydrolase 26 antibody
    • Ubiquitin carboxyl-terminal hydrolase 26 antibody
    • Ubiquitin specific peptidase 26 antibody
    • Ubiquitin specific processing protease 26 antibody
    • Ubiquitin specific protease 26 antibody
    • Ubiquitin thioesterase 26 antibody
    • Ubiquitin thiolesterase 26 antibody
    • Ubiquitin-specific-processing protease 26 antibody
    • UBP26_HUMAN antibody
    • USP 26 antibody
    • USP26 antibody
    see all

Anti-USP26 antibody images

  • All lanes : Anti-USP26 antibody (ab101650) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with immunising peptide at 1 µg/ml
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 94 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab101650 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101650 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-USP26 antibody (ab101650)

This product has been referenced in:
  • Wosnitzer MS  et al. Ubiquitin Specific Protease 26 (USP26) Expression Analysis in Human Testicular and Extragonadal Tissues Indicates Diverse Action of USP26 in Cell Differentiation and Tumorigenesis. PLoS One 9:e98638 (2014). IHC-P, IHC-Fr ; Human . Read more (PubMed: 24922532) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Human Cell lysate - whole cell (U2OS-osteosarcoma)
Specification U2OS-osteosarcoma
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Carlos le Sage

Verified customer

Submitted Nov 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"